亚硝酸盐和硝酸盐对绿藻叶绿素荧光的影响。

Effect of nitrite and nitrate on chlorophyll fluorescence in green algae.

机构信息

Institute of Molecular Biophysics, Florida State University, 32306, Tallahassee, Florida, USA.

出版信息

Planta. 1973 Mar;111(1):41-6. doi: 10.1007/BF00386733.

Abstract

The influence of nitrite and nitrate on chlorophyll fluorescence, a very sensitive indicator for the redox state of the primary acceptor of photosystem II of photosynthesis, was studied in green algae (several species of Chlorella, and Ankistrodesmus braunii). In phosphate solution under an atmosphere of nitrogen (i.e., in the absence of O2 and CO2, and without nitrite or nitrate), fluorescence shows a pronounced induction and then rises to a high steady-state level. In the presence of nitrite, however, fluorescence decreases after a rather short induction peak to a much lower steady-state. Nitrate, on the other hand, does not have any influence on either induction or steady-state of fluorescence. These results clearly demonstrate that nitrite reduction in the light is very closely coupled to the photosynthetic electron transport system, whereas nitrate is not reduced photosynthetically in vivo.

摘要

研究了亚硝酸盐和硝酸盐对叶绿素荧光的影响，叶绿素荧光是光合作用中光系统 II 原初受体氧化还原状态的一个非常敏感的指标。在含磷酸盐的溶液中，在氮气气氛下（即没有 O2 和 CO2，也没有亚硝酸盐或硝酸盐），荧光表现出明显的诱导，然后上升到一个高的稳态水平。然而，在存在亚硝酸盐的情况下，荧光在一个相当短的诱导峰后下降到一个低得多的稳态。另一方面，硝酸盐对荧光的诱导和稳态都没有影响。这些结果清楚地表明，在光照下亚硝酸盐的还原与光合作用电子传递系统密切相关，而硝酸盐在体内不能被光合作用还原。