Botany Department, University of Adelaide, 5001, S.A., Australia.
Photosynth Res. 1983 Jan;4(1):213-27. doi: 10.1007/BF00041817.
Characteristics of primary phases in chlorophyll a fluorescence transients based on room temperature in vivo measurement with a Plant Productivity Fluorometer (Brancker Model SF-10) can be greatly facilitated by coupling the instrument to a fast data acquisition system. The SF-10 was linked to a Multitech Industrial Corporation Microprofessor Microcomputer and further modified to ensure simultaneous onset of light activation and signal capture. Circuit diagrams and program listings are given in detail.This microprocessor system is capable of capturing signal changes over a minimum period of 200 milliseconds to a maximum of 6 seconds. Accuracy of recorded data is dependent on rate of change of the input signal and the recording time period. Acquisition and storage of 5000 points from zero to 300 milliseconds ensured clear resolution of Fo, I and D when played back over 120 seconds on a chart recorder. For routine use, the primary transient can be captured over 0-2 seconds and then replayed as an accompaniment to standard slower presentation of primary plus secondary transients. Coincidence of signal amplitude for Fp on both systems can then be ascertained while retaining adequate resolution of Fo and I.
通过将仪器与快速数据采集系统相耦合，可以极大地促进基于室温活体测量的叶绿素 a 荧光瞬变的主要阶段的特征研究，该仪器是用植物生产力荧光计（Brancker 型号 SF-10）完成的。SF-10 与 Multitech 工业公司的 Microprofessor 微电脑相连，并进一步进行了修改，以确保光激活和信号捕获同时开始。详细给出了电路图和程序清单。这个微处理器系统能够在最小 200 毫秒到最大 6 秒的时间段内捕获信号变化。记录数据的准确性取决于输入信号的变化率和记录时间段。从 0 到 300 毫秒采集和存储 5000 个点，在记录器上回放 120 秒以上时，可以清楚地区分 Fo、I 和 D。对于常规使用，可以在 0-2 秒内捕获主要瞬变，然后将其作为主要瞬变和次要瞬变的标准较慢呈现的伴奏重播。同时保留 Fo 和 I 的足够分辨率，可以确定两个系统上 Fp 的信号幅度的一致性。
