Department of Agricultural Biochemistry, School of Agriculture, University of Edinburgh, West Mains Road, EH9, Edinburgh, U.K..
Planta. 1973 Jun;114(2):195-8. doi: 10.1007/BF00387476.
The activity of starch synthetase in developing barley endosperm was measured in amyloplasts and in the soluble endosperm fraction by incorporation of radioactively labelled glucose into starch. Both uridine diphosphate glucose (UDPG) and adenosine diphophate glucose (ADPG) were used as glucosyl donors. Enzyme activity was initially located in the soluble fraction, but increasing activity could be detected in the amyloplast fraction during endosperm maturation.
采用放射性标记葡萄糖掺入淀粉的方法，测量了发育中的大麦胚乳中淀粉合酶的活性。两种葡萄糖基供体均使用尿苷二磷酸葡萄糖（UDPG）和腺苷二磷酸葡萄糖（ADPG）。酶活性最初位于可溶部分，但在胚乳成熟过程中，可在淀粉体部分检测到不断增加的活性。
