Department of Biochemistry and Biophysics, University of California, Davis, California 95616.
Plant Physiol. 1971 Dec;48(6):765-9. doi: 10.1104/pp.48.6.765.
alpha-1,4-glucan alpha-4-glucosyl-transferases were extracted from kernels of waxy maize harvested 22 days after pollination and separated by gradient elution from a diethylaminoethyl-cellulose column. Both fractions could utilize amylopectin, amylose, glycogen, maltotriose and maltose as primers. The rate of glucose transfer from adenosine diphosphoglucose to rabbit liver glycogen of fraction II was 78% of the rate of glucose transfer to amylopectin, but with fraction I the rate of transfer of glucose to rabbit liver glycogen was 380% of that observed to amylopectin. Glucan synthesis in the absence of added primer was found in fraction I in the presence of 0.5 m sodium citrate and bovine serum albumin. The unprimed product was a methanol-precipitable glucan with principally alpha-1,4 linkages and some alpha-1,6 linkages, and its iodine spectrum was similar to that of amylopectin.
α-1,4-葡聚糖α-4-葡萄糖基转移酶从授粉后 22 天收获的蜡质玉米的胚乳中提取，并通过从 DEAE-纤维素柱上进行梯度洗脱进行分离。这两个级分都可以利用支链淀粉、直链淀粉、糖原、麦芽三糖和麦芽糖作为引物。第二级分从腺苷二磷酸葡萄糖向兔肝糖原的葡萄糖转移率为转移至支链淀粉的葡萄糖转移率的 78%，但第一级分向兔肝糖原的葡萄糖转移率为观察到的支链淀粉的 380%。在 0.5m 柠檬酸钠和牛血清白蛋白存在下，在第一级分中发现了在没有添加引物的情况下的葡聚糖合成。未加引物的产物是一种甲醇可沉淀的葡聚糖，主要具有α-1,4 键和一些α-1,6 键，其碘谱与支链淀粉相似。
