一种伴侣蛋白作为原子转移自由基聚合的蛋白质纳米反应器。

A chaperonin as protein nanoreactor for atom-transfer radical polymerization.

机构信息

Department of Chemistry, University of Basel, Klingelbergstrasse 80, 4056 Basel (Switzerland).

出版信息

Angew Chem Int Ed Engl. 2014 Jan 27;53(5):1443-7. doi: 10.1002/anie.201306798. Epub 2013 Dec 20.

DOI:10.1002/anie.201306798

Abstract

The group II chaperonin thermosome (THS) from the archaea Thermoplasma acidophilum is reported as nanoreactor for atom-transfer radical polymerization (ATRP). A copper catalyst was entrapped into the THS to confine the polymerization into this protein cage. THS possesses pores that are wide enough to release polymers into solution. The nanoreactor favorably influenced the polymerization of N-isopropyl acrylamide and poly(ethylene glycol)methylether acrylate. Narrowly dispersed polymers with polydispersity indices (PDIs) down to 1.06 were obtained in the protein nanoreactor, while control reactions with a globular protein-catalyst conjugate only yielded polymers with PDIs above 1.84.

摘要

嗜热菌 Thermoplasma acidophilum 的 II 组分子伴侣热体（THS）被报道为原子转移自由基聚合（ATRP）的纳米反应器。铜催化剂被包埋在 THS 中，将聚合限制在这个蛋白质笼内。THS 具有足够宽的孔，可将聚合物释放到溶液中。纳米反应器有利于 N-异丙基丙烯酰胺和聚（乙二醇）甲醚丙烯酸酯的聚合。在蛋白质纳米反应器中得到了分散度指数（PDI）低至 1.06 的窄分散聚合物，而用球形蛋白-催化剂缀合物进行的对照反应仅得到 PDI 高于 1.84 的聚合物。