Solt D B, Polverini P J, Calderon L
Department of Pathology, Northwestern University Dental School, Chicago, Illinois.
J Oral Pathol. 1987 Jul;16(6):294-302. doi: 10.1111/j.1600-0714.1987.tb00697.x.
In order to assess the relative carcinogenic potency of polycyclic aromatic hydrocarbons in hamster buccal pouch, groups of male Syrian golden hamsters were treated by painting the buccal pouch surfaces for up to 20 weeks with equimolar concentrations of 7, 12-dimethylbenzanthracene; benzanthracene, 3,4-benzpyrene; or 20-methylcholanthrene dissolved in paraffin oil. Control hamsters were simarily treated with paraffin oil. Whereas 100% of the 7, 12-dimethylbenzanthracene treated hamsters developed buccal pouch carcinomas within the 20-week treatment period, no cancers were observed in the control hamsters or in those treated with the other polycyclic aromatic hydrocarbons. Simarily, of the various treatment groups, only 7,12-dimethylbenzanthracene treated hamsters displayed the efficient induction of foci of intense gamma glutamyltranspeptidase (GGT) histochemical activity within the buccal pouch epithelium. These results support the working hypothesis that induction of GGT foci is an early indicator of developing carcinoma in this experimental model.
为了评估多环芳烃在仓鼠颊囊中的相对致癌效力，将雄性叙利亚金黄仓鼠分组，用等摩尔浓度的7,12 - 二甲基苯并蒽、苯并蒽、3,4 - 苯并芘或20 - 甲基胆蒽溶解于石蜡油中，对颊囊表面进行涂抹处理，长达20周。对照仓鼠同样用石蜡油处理。在20周的治疗期内，100%经7,12 - 二甲基苯并蒽处理的仓鼠发生了颊囊癌，而对照仓鼠或用其他多环芳烃处理的仓鼠未观察到癌症。同样，在各个治疗组中，只有经7,12 - 二甲基苯并蒽处理的仓鼠在颊囊上皮内高效诱导出了强烈的γ - 谷氨酰转肽酶（GGT）组织化学活性灶。这些结果支持了一个工作假设，即在这个实验模型中，GGT灶的诱导是癌发生的早期指标。
