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蛋白激酶C同工酶α、βI、βII和γ在成年及发育中大鼠肾脏中的免疫定位

Immunolocalization of Protein Kinase C Isoenzymes α, βI, βII and γ in Adult and Developing Rat Kidney.

作者信息

Kim Wan-Young, Lee Gye-Sil, Kim Young-Hee, Park Eun-Young, Hwang Jin-Sun, Kim Hyang, Kim Jin

机构信息

Department of Anatomy and MRC for Cell Death Disease Research Center, The Catholic University of Korea College of Medicine, Seoul, Korea.

Department of Internal Medicine, Sungkyunkwan University, Kangbuk Samsung Hospital, Seoul, Korea.

出版信息

Electrolyte Blood Press. 2007 Dec;5(2):75-88. doi: 10.5049/EBP.2007.5.2.75. Epub 2007 Dec 31.

DOI:10.5049/EBP.2007.5.2.75
PMID:24459505
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3894519/
Abstract

Protein kinase C (PKC) plays an important role not only in signal transduction mechanisms in various biological processes, but also in the regulation of growth and differentiation during development. We studied the classical PKCα, βI, βII and γ, with regard to their expression in adult and developing rat kidney. PKCα appeared in the ureteric bud at embryonic day (E) 16, and the proximal and distal anlage at E18. After birth, the immunoreactivity of PKCα gradually decreased. In adult, PKCα was expressed intensely in the connecting tubule (CNT), the collecting ducts (CD) and the renal corpuscle, and weakly in the proximal and distal tubules. PKCβI appeared in the ureteric bud at E16, and the proximal anlage at E18. After birth, the immunoreactivity of PKCβI gradually disappeared from the CD and proximal tubule. In adult, PKCβI was expressed in the intercalated cells of the CNT and cortical CD, the proximal straight tubule, and the renal corpuscle. PKCβII appeared in distal anlage at E18, and increased markedly after birth. In the CD, PKCβII immunoreactivity appeared after birth. In adult, PKCβII was expressed in the distal tubule, the CNT and the CD. The immunoreactivity for PKCγ appeared only in the proximal anlage at E18, and increased temporally around the time of birth. However, no immunoreactivity for PKCγ was observed in adult rat kidney. These results indicate that classical PKC isoforms appear to play a role in the regulation of various renal functions and differentiation within specific functional units of the uriniferous tubule in rat kidney.

摘要

蛋白激酶C（PKC）不仅在各种生物过程的信号转导机制中发挥重要作用，而且在发育过程中的生长和分化调节中也起重要作用。我们研究了经典的PKCα、βI、βII和γ在成年和发育中的大鼠肾脏中的表达情况。PKCα在胚胎第16天出现在输尿管芽中，在第18天出现在近端和远端原基中。出生后，PKCα的免疫反应性逐渐降低。在成年大鼠中，PKCα在连接小管（CNT）、集合管（CD）和肾小体中强烈表达，在近端和远端小管中弱表达。PKCβI在胚胎第16天出现在输尿管芽中，在第18天出现在近端原基中。出生后，PKCβI的免疫反应性逐渐从集合管和近端小管中消失。在成年大鼠中，PKCβI在连接小管和皮质集合管的闰细胞、近端直小管和肾小体中表达。PKCβII在第18天出现在远端原基中，出生后明显增加。在集合管中，PKCβII的免疫反应性在出生后出现。在成年大鼠中，PKCβII在远端小管、连接小管和集合管中表达。PKCγ的免疫反应性仅在第18天出现在近端原基中，并在出生前后暂时增加。然而，在成年大鼠肾脏中未观察到PKCγ的免疫反应性。这些结果表明，经典的PKC同工型似乎在大鼠肾脏泌尿小管特定功能单位内的各种肾功能调节和分化中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/3265c501a267/ebp-5-75-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/ce1858f5ec50/ebp-5-75-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/97c0bc759550/ebp-5-75-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/9e58db6f27c4/ebp-5-75-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/23d10296324b/ebp-5-75-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/5054dcb46c92/ebp-5-75-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/bd1af39913b8/ebp-5-75-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/92e9e41d7a5a/ebp-5-75-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/b92d7096e6cb/ebp-5-75-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/3265c501a267/ebp-5-75-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/ce1858f5ec50/ebp-5-75-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/97c0bc759550/ebp-5-75-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/9e58db6f27c4/ebp-5-75-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/23d10296324b/ebp-5-75-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/5054dcb46c92/ebp-5-75-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/bd1af39913b8/ebp-5-75-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/92e9e41d7a5a/ebp-5-75-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/b92d7096e6cb/ebp-5-75-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/3894519/3265c501a267/ebp-5-75-g009.jpg

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