Fukuzaki A, Kaneto H, Ikeda S, Orikasa S
Department of Urology, Tohoku University School of Medicine, Sendai, Japan.
Tohoku J Exp Med. 1996 Mar;178(3):263-9. doi: 10.1620/tjem.178.263.
Protein kinase C (PKC), the calcium and phospholipid-dependent kinase, is a key enzyme for regulation of growth and differentiation of various cells and tissues. Abnormalities of PKC modulation may cause diseases such as neoplasia. The present study was designed to demonstrate localization of PKC in normal human nephron segments. Surgically extirpated specimens for renal tumor were used. PKC was determined by immunoblotting and immunohistochemical methods using specific antibody against PKC that recognizes alpha and beta subtypes of this enzyme. Immunoblotting study demonstrated PKC in the cytosolic fractions of homogenized cortical tubules and medullary tubules prepared from morphologically normal part of the kidney. PKC was faintly detected in glomerular supernatants. Immunohistochemical study revealed that PKC was present throughout the nephron and especially concentrated in the cytoplasm of the proximal tubules and the medullary collecting ducts. The results indicate that PKC appears to play a role in regulation of normal nephron function in human kidney.
蛋白激酶C（PKC）是一种钙和磷脂依赖性激酶，是调节各种细胞和组织生长与分化的关键酶。PKC调节异常可能导致诸如肿瘤形成等疾病。本研究旨在证明PKC在正常人类肾单位各节段中的定位。使用手术切除的肾肿瘤标本。采用针对PKC的特异性抗体，通过免疫印迹和免疫组织化学方法测定PKC，该抗体可识别该酶的α和β亚型。免疫印迹研究表明，在从肾脏形态学正常部位制备的皮质肾小管和髓质肾小管匀浆的胞质部分中存在PKC。在肾小球上清液中可微弱检测到PKC。免疫组织化学研究显示，PKC存在于整个肾单位中，尤其集中在近端小管和髓质集合管的细胞质中。结果表明，PKC似乎在人类肾脏正常肾单位功能的调节中发挥作用。
