The Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, 35 Qinghua Dong Road, Haidian District, Beijing 100083, P. R. China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, P. R. China.
Z Naturforsch C J Biosci. 2013 Sep-Oct;68(9-10):416-27.
The legume pod borer, Maruca vitrata (Lepidoptera: Crambidae; syn. M. testulalis), is a serious pantropical insect pest of grain legumes. Comparative studies of M. vitrata female sex pheromone components in two different geographic populations in China, Wuhan and Huazhou, confirmed that (E,E)-10,12-hexadecadienal (E10,E12-16:Ald) and (E)-10-hexadecenal (E10-16:Ald) were present in variable ratios in all pheromone gland extracts of both populations. (E,E)-10,12-hexadecadienol (E10,E12-16:OH) was always detected in minor amounts using polar DB-WAX columns, but was never detected using medium-polar DB-17 columns for the two populations. E10-16:OH was not found in any of the M. vitrata sex pheromone gland extracts. The average ratios of E10-16:Ald, E10,E12-16:Ald, and E10,E12-16:OH in the pheromone gland extracts of populations from Wuhan and Huazhou were 79.5:100:12.1 and 10.3:100:0.7, respectively. Electrophysiological testing suggested that E10,E12-16:Ald elicited the highest male electroantennography (EAG) response, followed by E10,E12-16:OH and E10-16:Ald. Field-trapping tests with single synthetic sex pheromone lures showed that E10,E12-16:OH alone could not attract males, whereas E10,E12-16:Ald or E10-16:Ald alone attracted few males. Wuhan and Huazhou males were most attracted by lures containing E10-16:Ald + E10,E12-16:Ald + E10,E12-16:OH in doses of (80 + 100 + 10) microg and (10 + 100 + 10) microg, respectively, per vial. Males could discriminate between the blends that were most attractive to their own geographic population and those that were most attractive to the reference population. Our findings suggest that geographic variation exists in the sex pheromone system of M. vitrata in China. The results are discussed with regard to the mechanisms underlying the sex pheromone variation.
豆荚，豆科植物豆荚螟，是一种严重的泛热带昆虫害虫的粮食豆类。比较研究豆荚螟女性性信息素成分在两个不同的地理种群在中国，武汉和化州，证实（E，E）-10,12-十六碳二烯醛（E10，E12-16：Ald）和（E）-10-十六碳烯醛（E10-16：Ald）存在于可变比例的所有信息素腺提取物的两个种群。（E，E）-10,12-十六碳二烯醇（E10，E12-16：OH）总是用极性 DB-WAX 柱检测到少量，但从未在两个种群的中等极性 DB-17 柱上检测到。E10-16：OH 在任何豆荚螟性信息素腺提取物中都没有发现。平均比例的 E10-16：Ald，E10，E12-16：Ald和 E10，E12-16：OH 在信息素腺提取物中的人口从武汉和化州分别为 79.5:100:12.1 和 10.3:100:0.7。电生理测试表明，E10，E12-16：Ald 引起最高的雄性触角电图（EAG）反应，其次是 E10，E12-16：OH 和 E10-16：Ald。单合成性信息素诱饵的田间诱捕试验表明，E10，E12-16：OH 单独不能吸引雄性，而 E10，E12-16：Ald 或 E10-16：Ald 单独吸引很少的雄性。武汉和化州的雄性最受含有 E10-16：Ald+E10，E12-16：Ald+E10，E12-16：OH 的诱饵的吸引，剂量分别为（80+100+10）μg 和（10+100+10）μg 每个小瓶。雄性可以区分最吸引其自身地理种群的混合物和最吸引参考种群的混合物。我们的研究结果表明，在中国豆荚螟的性信息素系统存在地理变异。结果与性信息素变异的机制有关。
