阿派低脉冲场图谱分型质量差的克服报告及李斯特菌脉冲场凝胶电泳技术的简短回顾。

Report on overcoming the poor quality of Apai pulsotypes with a short review on PFGE for listeria monocytogenes.

机构信息

Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.

出版信息

Pol J Microbiol. 2013;62(3):307-9.

Abstract

Since listeriosis, caused by Listeria monocytogenes, is one of the important concerns of public health in Europe related to foodborne zoonoses, an efficient protocol for isolate typing is necessary when performing epidemiological studies. Three standardized PFGE protocols available for L. monocytogenes were briefly reviewed. Since observing a poor-quality of ApaI pulsotypes in our laboratory, enzymes from three different manufacturers were compared. The obtained pulsotypes showed that restriction digestion with ApaI from New England BioLabs should be complemented with a subsequent overnight incubation of PFGE plugs in TE buffer for better performance.

摘要

由于李斯特菌病是由单增李斯特菌引起的，是欧洲与食源性病原体相关的重要公共卫生问题之一，因此在进行流行病学研究时，需要有效的分离株分型方法。本文简要综述了三种用于单增李斯特菌的标准化 PFGE 方案。由于在本实验室观察到 ApaI 脉冲类型质量较差，因此比较了来自三个不同制造商的酶。所得的脉冲类型表明，用来自 New England BioLabs 的 ApaI 进行限制消化后，应在 TE 缓冲液中过夜孵育 PFGE 插头，以获得更好的性能。