Lozniewski A, Humbert A, Corsaro D, Schwartzbrod J, Weber M, Le Faou A
Laboratoire de Bactériologie-Virologie, Faculté de Médecine, Université Henri-Poincaré Nancy, France.
Lett Appl Microbiol. 2001 May;32(5):336-9. doi: 10.1046/j.1472-765x.2001.00918.x.
Listeria monocytogenes strains isolated in the same geographical area from sewage sludge and from patients presenting with listeriosis were compared.
All isolates were typed by serotyping, phage typing and SmaI/ApaI pulsed-field gel electrophoresis (PFGE). Among the sludge isolates (n=32), 22 subtypes could be distinguished by the combination of all typing methods. The human isolates (n=11) were distributed into 10 subtypes which clearly differed from those observed among sludge isolates, except for one cluster formed by two related human isolates which showed high similarity in PFGE patterns (SmaI: 92%; ApaI: 89.5%) with one sludge isolate.
These results suggest the existence of an epidemiological link between sludge and human isolates, but they may also be reflecting the distribution of L. monocytogenes types within the environment.
Sludge and human L. monocytogenes may be related but further epidemiological studies are necessary to elucidate this point.
比较从同一地理区域的污水污泥和患李斯特菌病患者中分离出的单核细胞增生李斯特菌菌株。
所有分离株均通过血清分型、噬菌体分型和SmaI/ApaI脉冲场凝胶电泳（PFGE）进行分型。在污泥分离株（n = 32）中，通过所有分型方法的组合可区分出22个亚型。人类分离株（n = 11）分为10个亚型，这些亚型与污泥分离株中观察到的亚型明显不同，除了由两个相关人类分离株形成的一个簇，该簇在PFGE图谱（SmaI：92%；ApaI：89.5%）上与一个污泥分离株显示出高度相似性。
这些结果表明污泥和人类分离株之间存在流行病学联系，但它们也可能反映了环境中单核细胞增生李斯特菌类型的分布。
污泥和人类单核细胞增生李斯特菌可能相关，但需要进一步的流行病学研究来阐明这一点。
