单核细胞增生李斯特菌的污泥分离株与临床分离株的比较。

Comparison of sludge and clinical isolates of Listeria monocytogenes.

作者信息

Lozniewski A, Humbert A, Corsaro D, Schwartzbrod J, Weber M, Le Faou A

机构信息

Laboratoire de Bactériologie-Virologie, Faculté de Médecine, Université Henri-Poincaré Nancy, France.

出版信息

Lett Appl Microbiol. 2001 May;32(5):336-9. doi: 10.1046/j.1472-765x.2001.00918.x.

DOI:10.1046/j.1472-765x.2001.00918.x

PMID:11328501

Abstract

AIMS

Listeria monocytogenes strains isolated in the same geographical area from sewage sludge and from patients presenting with listeriosis were compared.

METHODS AND RESULTS

All isolates were typed by serotyping, phage typing and SmaI/ApaI pulsed-field gel electrophoresis (PFGE). Among the sludge isolates (n=32), 22 subtypes could be distinguished by the combination of all typing methods. The human isolates (n=11) were distributed into 10 subtypes which clearly differed from those observed among sludge isolates, except for one cluster formed by two related human isolates which showed high similarity in PFGE patterns (SmaI: 92%; ApaI: 89.5%) with one sludge isolate.

CONCLUSION

These results suggest the existence of an epidemiological link between sludge and human isolates, but they may also be reflecting the distribution of L. monocytogenes types within the environment.

SIGNIFICANCE AND IMPACT OF THE STUDY

Sludge and human L. monocytogenes may be related but further epidemiological studies are necessary to elucidate this point.

摘要

目的

比较从同一地理区域的污水污泥和患李斯特菌病患者中分离出的单核细胞增生李斯特菌菌株。

方法与结果

所有分离株均通过血清分型、噬菌体分型和SmaI/ApaI脉冲场凝胶电泳（PFGE）进行分型。在污泥分离株（n = 32）中，通过所有分型方法的组合可区分出22个亚型。人类分离株（n = 11）分为10个亚型，这些亚型与污泥分离株中观察到的亚型明显不同，除了由两个相关人类分离株形成的一个簇，该簇在PFGE图谱（SmaI：92%；ApaI：89.5%）上与一个污泥分离株显示出高度相似性。