Pan Changjiang, Nie Yudong, Ding Hongyan, Dong Yunxiao
Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology, Huai'an 223003, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2013 Oct;30(5):1044-51.
The main purpose of the present study was to investigate the cell behaviors of rat primary osteoblast cells on extracellular matrix (ECM) protein micropatterns. For this purpose, a series of fibronectin micropatterns with different shapes and varying dimensions were created on polystyrene (PS) surfaces by microcontact printing. The results of confocal laser scanning microscopy (CLSM) images indicated that excellent micropatterns were successfully obtained. These protein patterns were stable during the cell culture. The cell experiments suggested that the osteoblast cells preferentially attached onto protein-functionalized areas and displayed different cell shape and spreading behavior on different protein micropatterns. The protein micropatterns can significantly influence the cell adhesion, spreading, alignment and orientation and so on. Therefore, this work can be used to modify biomaterial surfaces, especially that of bone-implant biomaterials, to effectively control cell behavior. It further contributes to clarify the interfacial biological behaviors between biomaterials and osteoblast and can provide the cues for development of bone implantable materials which is able to modulate osteoblast cell growth behavior.
本研究的主要目的是研究大鼠原代成骨细胞在细胞外基质（ECM）蛋白微图案上的细胞行为。为此，通过微接触印刷在聚苯乙烯（PS）表面创建了一系列具有不同形状和不同尺寸的纤连蛋白微图案。共聚焦激光扫描显微镜（CLSM）图像结果表明成功获得了优良的微图案。这些蛋白质图案在细胞培养过程中是稳定的。细胞实验表明，成骨细胞优先附着在蛋白质功能化区域，并在不同的蛋白质微图案上表现出不同的细胞形状和铺展行为。蛋白质微图案可显著影响细胞粘附、铺展、排列和取向等。因此，这项工作可用于修饰生物材料表面，尤其是骨植入生物材料的表面，以有效控制细胞行为。它进一步有助于阐明生物材料与成骨细胞之间的界面生物学行为，并可为能够调节成骨细胞生长行为的骨可植入材料的开发提供线索。
