Sharma Vishal, Köllmer Melanie, Szymusiak Magdalena, Nitsche Ludwig C, Gemeinhart Richard A, Liu Ying
Department of Chemical Engineering and §Department of Bioengineering, University of Illinois at Chicago , Chicago, Illinois 60607, United States.
Biomacromolecules. 2014 Mar 10;15(3):756-62. doi: 10.1021/bm401550r. Epub 2014 Feb 11.
Heterogeneous toroidal-spiral particles (TSPs) were generated by polymer droplet sedimentation, interaction, and cross-linking. TSPs provide a platform for encapsulation and release of multiple compounds of different sizes and physicochemical properties. As a model system, we demonstrate the encapsulation and independently controlled release of an anti-VEGFR-2 antibody and irinotecan for the treatment of glioblastoma multiforme. The anti-VEGFR-2 antibody was released from the TS channels and its binding to HUVECs was confirmed by confocal microscopy and flow cytometry, suggesting active antibody encapsulation and release. Irinotecan, a small molecule drug, was released from the dense polymer matrix of poly(ethylene glycol) diacrylate (MW ~ 700 g/mol; PEGDA 700). Released irinotecan inhibited the proliferation of U251 malignant glioma cells. Since the therapeutic compounds are released through different pathways, specifically diffusion through the polymer matrix versus TS channels, the release rate can be controlled independently through the design of the structure and material of particle components.
