Zhang Shan-Chun, Jin Wen, Liu Hui, Jin Ming-Juan, Chen Ze-Xin, Ding Zhe-Yuan, Zheng Shuang-Shuang, Wang Li-Juan, Yu Yun-Xian, Chen Kun
Department of Epidemiology and Health Statistics, School of Public Health, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China E-mail :
Asian Pac J Cancer Prev. 2013;14(12):7127-31. doi: 10.7314/apjcp.2013.14.12.7127.
The primary aim of this study was to evaluate the relationship of single nucleotide polymorphisms (SNPs) in ribosomal protein SA (RPSA) gene with colorectal cancer (CRC). A case-control study including 388 controls and 387 patients with CRC was conducted in a Chinese population. Information about socio-demography and living behavior factors was collected by a structured questionnaire. Three SNPs (rs2133579, rs2269349, rs7641291) in RPSA gene were genotyped by Illumina SnapShot method. Multiple logistic regression models were used for assessing the joint effects between tea consumption and SNPs on CRC. The subjects with rs2269349 CC genotype had a decreased risk for CRC (OR=0.60; 95%CI = 0.37-0.99), compared with TT/CT genotype after adjustment for covariates. A similar association of rs2269349 with rectal cancer was observed (OR=0.49; 95%CI=0.24-1.00). Further analyses indicated that this SNP could modify the protective effect of tea drinking on CRC. Among the subjects with rs2269349 TT/CT or rs2133579 AA/GA, there was a marginal significantly lower risk of CRC (OR and 95%CI: 0.63 and 0.39-1.01 for rs2269349; 0.64 and 0.40-1.02 for rs2133579) in tea-drinking subjects in comparison to non-tea-drinking subjects. Mutants in the RPSA gene might be associated with genetic susceptibility to CRC and influence the protective effect of tea consumption in the Chinese population.
本研究的主要目的是评估核糖体蛋白SA（RPSA）基因中的单核苷酸多态性（SNP）与结直肠癌（CRC）的关系。在中国人群中开展了一项病例对照研究，纳入388名对照者和387例CRC患者。通过结构化问卷收集社会人口统计学和生活行为因素信息。采用Illumina SnapShot方法对RPSA基因中的3个SNP（rs2133579、rs2269349、rs7641291）进行基因分型。使用多重逻辑回归模型评估饮茶与SNP对CRC的联合作用。校正协变量后，与TT/CT基因型相比，rs2269349 CC基因型的受试者患CRC的风险降低（OR=0.60；95%CI=0.37-0.99）。观察到rs2269349与直肠癌存在类似关联（OR=0.49；95%CI=0.24-1.00）。进一步分析表明，该SNP可改变饮茶对CRC的保护作用。在rs2269349 TT/CT或rs2133579 AA/GA的受试者中，饮茶者患CRC的风险略低于不饮茶者（rs2269349的OR及95%CI：0.63和0.39-1.01；rs2133579的OR及95%CI：0.64和0.40-1.02）。RPSA基因中的突变可能与CRC的遗传易感性相关，并影响中国人群中饮茶的保护作用。
