Bao Shan, Yang Shu-Ying, Li Zhuo-Ri, Wen Ge-Bo
Department of Endocrinology, The First Affiliated Hospital, University of South China, Hengyang 421001, China; Department of Gynecology and Obstetrics, Hainan Provincial People's Hospital, Haikou 570311, China.
Department of Gynecology and Obstetrics, Hainan Provincial People's Hospital, Haikou 570311, China.
Asian Pac J Trop Med. 2014 Feb;7(2):149-52. doi: 10.1016/S1995-7645(14)60012-2.
To screen, identify, and compare the serum biomarkers between anovulatory dysfunctional uterine bleeding (ADUB) and ovulatory dysfunctional uterine bleeding (ODUB) in Lizu females.
The subjects included 128 ADUB patients, 63 ODUB patients, and 93 controls. The serum and supernate of the subjects' mense were collected and stored at -80 °C until use. Differential proteins in the sera of three groups were screened using surface-enhanced laser desorption ionization time-of-flight mass spectrometry. The screened proteins were then identified by tricine-SDS-PAGE gel and spectrometry. Protein expression levels in the menses of ADUB, ODUB, and control subjects were determined using ELISA, RT-PCR, and Western blotting. SPSS 14.1 was used for statistical analysis and chart drawing (α = 0.05).
Three differential protein peaks with peak values of 11.80, 13.59, and 14.68 km/z were screened and identified as serum amyploid protein A (SAA), vascular endothelial growth factor, and vitamin K epoxide reductase, respectively. The SAA was highly expressed in the menses of ADUB and ODUB patients but poorly expressed in the controls. The vascular endothelial growth factor was highly expressed in the menses of ODUB and controls but poorly expressed in ADUB patients. Meanwhile, the vitamin K epoxide reductase was highly expressed in the menses of ADUB and control subjects but poorly expressed in ODUB patients.
The SAA is the common serum biomarker of ADUB and ODUB. ADUB may be related to angiogenesis impairment, whereas ODUB may be associated with blood coagulation disruption.
筛选、鉴定并比较黎族女性无排卵性功能失调性子宫出血（ADUB）和有排卵性功能失调性子宫出血（ODUB）之间的血清生物标志物。
研究对象包括128例ADUB患者、63例ODUB患者和93例对照者。收集研究对象月经期间的血清和上清液，储存于-80℃备用。采用表面增强激光解吸电离飞行时间质谱法筛选三组血清中的差异蛋白。然后通过三羟甲基氨基甲烷-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（tricine-SDS-PAGE）凝胶和光谱法鉴定筛选出的蛋白质。采用酶联免疫吸附测定（ELISA）、逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测ADUB、ODUB和对照者月经期间的蛋白质表达水平。使用SPSS 14.1进行统计分析和图表绘制（α=0.05）。
筛选出三个差异蛋白峰，其峰值分别为11.80、13.59和14.68 km/z，分别鉴定为血清淀粉样蛋白A（SAA）、血管内皮生长因子和维生素K环氧化物还原酶。SAA在ADUB和ODUB患者月经期间高表达，而在对照者中低表达。血管内皮生长因子在ODUB和对照者月经期间高表达，而在ADUB患者中低表达。同时，维生素K环氧化物还原酶在ADUB和对照者月经期间高表达，而在ODUB患者中低表达。
SAA是ADUB和ODUB共同的血清生物标志物。ADUB可能与血管生成受损有关，而ODUB可能与凝血功能紊乱有关。
