阴道内装置中戊酸雌二醇和孕酮用量对采用定时人工授精方案处理的肉牛发情、排卵同步化及妊娠率的影响。
Effect of estradiol cypionate and amount of progesterone in the intravaginal device on synchronization of estrus, ovulation and on pregnancy rate in beef cows treated with FTAI based protocols.
作者信息
Uslenghi Gastón, Chaves Santiago González, Cabodevila Jorge, Callejas Santiago
机构信息
Postgraduate student, CONICET fellowship, Universidad Nacional del Centro de la Provincia de Buenos Aires, Facultad de Ciencias Veterinarias, Tandil, Argentina; Área de Reproducción, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina.
Veterinary, private activity in Grupo CONPAS, Rauch, Provincia de Buenos Aires, Argentina.
出版信息
Anim Reprod Sci. 2014 Feb;145(1-2):1-7. doi: 10.1016/j.anireprosci.2013.12.009. Epub 2014 Jan 7.
Three experiments were conducted to evaluate the effect of estradiol cypionate (ECP) and amount of progesterone in the intravaginal device (PID) on synchronization of estrus and ovulation, follicular dynamics, luteal dynamics and function and on pregnancy rate in beef cows treated with fixed-time artificial insemination (FTAI) based protocols. In Experiment 1, we evaluated the synchronization of ovulation using 1mg of ECP at PID removal (day 8 after PID insertion) or 1mg of estradiol benzoate (EB) 24h later, in cows treated with 0.558 or 1g of progesterone (P4). The final subgroups were: 0.558g+ECP: n=10; 0.558g+EB: n=11; 1g+ECP: n=10; 1g+EB: n=10. Ovarian ultrasonic examinations were performed to detect the dominant follicle and ovulation. There was no effect of treatments on the diameter of dominant follicle at any time, and on the mean interval to estrus and to ovulation (P>0.05); however, ECP treated cows had scattered distribution of estrus (P<0.03) and ovulation (P<0.03). In Experiment 2, cows received the following treatments: 0.558gP4+ECP: n=52; 0.558gP4+EB: n=52; 1gP4+ECP: n=50; 1gP4+EB: n=52; and FTAI. Pregnancy rate did not differ (P>0.05) between progesterone content (0.558g: 52.9%, 55/104; 1g: 56.9%, 58/102) but differed between estradiol esters (P<0.05; ECP: 48.9%, 49/102; EB: 61.5%, 64/104). In Experiment 3, cows received: 0.558gP4+ECP: n=55; 0.558gP4+EB: n=53; 1gP4+ECP: n=54; 1gP4+EB: n=53; and FTAI. Pregnancy rate did not differ (P>0.05) between progesterone content (0.558g: 48.1%, 52/108; 1g: 53.3%, 57/107) and estradiol esters (ECP: 47.7%, 52/109; EB: 53.8%, 57/106). In conclusion, ECP administration at device removal and progesterone content of PID has no influence on the synchronization of estrus, follicular dynamics, luteal dynamics and function. However, ECP administration affected the synchronization of ovulation and pregnancy rate in non-suckling beef cows, but did not affected pregnancy rate in suckling beef cows. Future studies should evaluate the distribution of ovulations in suckling Bos taurus beef cows.
进行了三项试验，以评估阴道内装置（PID）中孕烯醇酮（ECP）和孕酮含量对接受定时人工授精（FTAI）方案处理的肉牛发情和排卵同步化、卵泡动态、黄体动态及功能以及妊娠率的影响。在试验1中，我们在取出PID（插入PID后第8天）时使用1mg ECP或24小时后使用1mg苯甲酸雌二醇（EB），对分别使用0.558g或1g孕酮（P4）处理的母牛评估排卵同步化情况。最终亚组为：0.558g + ECP：n = 10；0.558g + EB：n = 11；1g + ECP：n = 10；1g + EB：n = 10。进行卵巢超声检查以检测优势卵泡和排卵情况。各处理在任何时间对优势卵泡直径、发情平均间隔时间和排卵平均间隔时间均无影响（P>0.05）；然而，接受ECP处理的母牛发情（P<0.03）和排卵（P<0.03）分布较为分散。在试验2中，母牛接受以下处理：0.558g P4 + ECP：n = 52；0.558g P4 + EB：n = 52；1g P4 + ECP：n = 50；1g P4 + EB：n = 52；以及FTAI。孕酮含量（0.558g：52.9%，55/104；1g：56.9%，58/102）之间的妊娠率无差异（P>0.05），但雌二醇酯之间存在差异（P<0.05；ECP：48.9%，49/102；EB：61.5%，64/104）。在试验3中，母牛接受：0.558g P4 + ECP：n = 55；0.558g P4 + EB：n = 53；1g P4 + ECP：n = 54；1g P4 + EB：n = 53；以及FTAI。孕酮含量（0.558g：48.1%，52/108；1g：53.3%，57/107）和雌二醇酯（ECP：47.7%，52/109；EB：53.8%，57/106）之间的妊娠率无差异（P>0.05）。总之，取出装置时给予ECP以及PID中的孕酮含量对发情同步化、卵泡动态、黄体动态及功能均无影响。然而，给予ECP影响了非哺乳肉牛的排卵同步化和妊娠率，但对哺乳肉牛的妊娠率无影响。未来研究应评估哺乳黄牛的排卵分布情况。
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