棕榈酰化对活细胞中 BKCa 通道扩散运动的影响。
Effects of palmitoylation on the diffusional movement of BKCa channels in live cells.
机构信息
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea.
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea; National Leading Research Laboratory, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea; Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea.
出版信息
FEBS Lett. 2014 Mar 3;588(5):713-9. doi: 10.1016/j.febslet.2014.01.014. Epub 2014 Jan 23.
BKCa channels are palmitoylated at a cluster of cysteine residues within the cytosolic linker connecting the 1st and 2nd transmembrane domains, and this lipid modification affects their surface expression. To verify the effects of palmitoylation on the diffusional dynamics of BKCa channels, we investigated their lateral movement. Compared to wild-type channels, the movement of mutant palmitoylation-deficient channels was much less confined and close to random. The diffusion of the mutant channel was also much faster than that of the wild type. Thus, the lateral movement of BKCa channels is greatly influenced by palmitoylation.
BKCa 通道在连接第一和第二跨膜结构域的胞质连接区内的一组半胱氨酸残基上发生棕榈酰化，这种脂修饰影响其表面表达。为了验证棕榈酰化对 BKCa 通道扩散动力学的影响，我们研究了它们的侧向运动。与野生型通道相比，突变的棕榈酰化缺陷型通道的运动受限制较小，更接近随机运动。突变通道的扩散也比野生型快得多。因此，BKCa 通道的侧向运动受棕榈酰化的影响很大。
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