Davis N S
Department of Urology and Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, New York.
Prostate. 1987;11(4):353-60. doi: 10.1002/pros.2990110407.
The finding of significant numbers of endocrine-paracrine (EP) cells in the prostate glands of guinea pigs and man suggests that these cells may be important in the regulation or modulation of prostatic function. Serotonin is a biogenic amine common to most prostatic EP cells. In order to extend current knowledge relating to these cells, an assay was developed using high-performance liquid chromatography to quantitate serotonin and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) in guinea pig and human prostatic tissue extracts. Levels of serotonin and 5-HIAA in the guinea pig whole-gland preparation were 105.4 +/- 70.6 ng/g and 48.4 +/- 95.7 ng/g, respectively. Normal human prostatic tissue contained 1423.9 +/- 750.8 ng/g serotonin and 66.7 +/- 92.8 ng/g 5-HIAA. Recoveries ranged between 60 and 100%. The detection limits were 24 pg/injection for serotonin and 12 pg/injection for 5-HIAA. This assay provides an expeditious, specific and highly sensitive method for the simultaneous determination of monoamines in guinea pig and human prostatic tissue.
在豚鼠和人类前列腺中发现大量内分泌旁分泌（EP）细胞，这表明这些细胞可能在前列腺功能的调节或调控中发挥重要作用。血清素是大多数前列腺EP细胞中常见的一种生物胺。为了拓展与这些细胞相关的现有知识，开发了一种利用高效液相色谱法定量豚鼠和人类前列腺组织提取物中血清素及其代谢物5-羟吲哚乙酸（5-HIAA）的检测方法。豚鼠全腺制剂中血清素和5-HIAA的水平分别为105.4±70.6 ng/g和48.4±95.7 ng/g。正常人类前列腺组织含有1423.9±750.8 ng/g血清素和66.7±92.8 ng/g 5-HIAA。回收率在60%至100%之间。血清素的检测限为24 pg/进样，5-HIAA的检测限为12 pg/进样。该检测方法为同时测定豚鼠和人类前列腺组织中的单胺提供了一种快速、特异且高度灵敏的方法。
