Hede A R, Berglund B G, Post C
Department of Clinical Pharmacology, University Hospital, Linköping, Sweden.
Pharmacol Toxicol. 1987 Sep;61(3):191-4. doi: 10.1111/j.1600-0773.1987.tb01801.x.
The effect of exposure to organic solvents on uptake and metabolism of 5-HT was studied in rat lung slices. It was found that under control conditions 5-HT was both taken up and metabolized to 5-HIAA. When halothane (35,000 ppm) or trichloroethylene (18,000 ppm) were equilibrated with the incubation medium the uptake of 5-HT decreased by approximately 50% after 30 min of incubation, and the production of 5-HIAA was inhibited by approximately 70% and 80%, respectively. The results are consistent with earlier studies using a much more elaborate technique, in which halothane and trichloroethylene were found to depress 5-HT uptake in isolated perfused rat lungs. Our results demonstrate that the simpler technique employing lung slices can also be used, to investigate factors affecting pulmonary uptake of endogenous amines, and, potentially, the uptake of other compounds as well.
在大鼠肺切片中研究了接触有机溶剂对5-羟色胺摄取和代谢的影响。发现在对照条件下，5-羟色胺既被摄取又代谢为5-羟吲哚乙酸。当将氟烷（35,000 ppm）或三氯乙烯（18,000 ppm）与孵育培养基平衡时，孵育30分钟后5-羟色胺的摄取量减少了约50%，5-羟吲哚乙酸的生成分别被抑制了约70%和80%。这些结果与早期使用更为精细技术的研究一致，在早期研究中发现氟烷和三氯乙烯可降低离体灌注大鼠肺中5-羟色胺的摄取。我们的结果表明，采用肺切片的更简单技术也可用于研究影响内源性胺类肺摄取的因素，并且潜在地也可用于研究其他化合物的摄取。
