Hede A R, Berglund B G, Post C
Department of Clinical Pharmacology, University Hospital, Linköping, Sweden.
Pharmacol Toxicol. 1987 Aug;61(2):138-41. doi: 10.1111/j.1600-0773.1987.tb01791.x.
Pulmonary uptake of 5-hydroxytryptamine (5-HT), zimeldine and propranolol were studied using the isolated perfused rat lung model. The 5-HT uptake was found to be attenuated by approximately 50 per cent in comparison to the control, when the lungs were ventilated with air containing 5,000 p.p.m. trichloroethylene. In experiments in which the active uptake of 5-HT was blocked with the selective 5-HT uptake inhibitor zimeldine (5 X 10(-6) M), the uptake of 5-HT decreased by 70 +/- 1.7 per cent (mean +/- S.E.M.). When trichloroethylene (5,000 p.p.m. and 18,000 p.p.m.) was added, no further decrease in uptake was noted. The uptake of 3H-zimeldine (10(-6) M) and 3H-propranolol (10(-6) M) was unaffected by ventilating the lungs with trichloroethylene. It is concluded that trichloroethylene inhibits the active uptake of 5-HT from the pulmonary circulation, but that it has no effect on the uptake of zimeldine or propranolol, which are taken up predominantly by passive diffusion.
使用离体灌注大鼠肺模型研究了5-羟色胺（5-HT）、齐美利定和普萘洛尔的肺摄取情况。当用含5000 ppm三氯乙烯的空气对肺进行通气时，发现5-HT摄取量与对照组相比降低了约50%。在使用选择性5-HT摄取抑制剂齐美利定（5×10⁻⁶ M）阻断5-HT主动摄取的实验中，5-HT摄取量降低了70±1.7%（平均值±标准误）。当加入三氯乙烯（5000 ppm和18000 ppm）时，未观察到摄取量进一步降低。用三氯乙烯对肺进行通气不影响³H-齐美利定（10⁻⁶ M）和³H-普萘洛尔（10⁻⁶ M）的摄取。结论是，三氯乙烯抑制5-HT从肺循环中的主动摄取，但对主要通过被动扩散摄取的齐美利定或普萘洛尔的摄取没有影响。
