• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大鼠骨骼肌中钠通道mRNA水平的调节

Modulation of sodium-channel mRNA levels in rat skeletal muscle.

作者信息

Cooperman S S, Grubman S A, Barchi R L, Goodman R H, Mandel G

机构信息

Department of Medicine, Tufts-New England Medical Center, Boston, MA 02111.

出版信息

Proc Natl Acad Sci U S A. 1987 Dec;84(23):8721-5. doi: 10.1073/pnas.84.23.8721.

DOI:10.1073/pnas.84.23.8721
PMID:2446331
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC299618/
Abstract

Action potentials in many types of excitable cells result from changes in permeability to Na ions. Although these permeability changes in nerve and muscle are mediated by voltage-gated Na channels that are functionally similar, we found that the Na-channel gene expressed in skeletal muscle is different from the genes coding for two Na channels (type I and type II) in brain. Despite the structural differences between muscle and brain Na-channel genes, a cDNA clone derived from rat brain hybridizes to skeletal muscle Na-channel mRNA of approximately 9.5 kilobases. We used this cDNA probe to measure changes in Na-channel mRNA levels in skeletal muscle during development and following denervation. By blot hybridization analysis of electrophoretically fractionated RNA, we found that Na-channel mRNA can be detected as early as embryonic day 17 and that mRNA levels increase 2-fold between birth and postnatal day 35. Denervation of adult muscle causes a further 2- to 3-fold increase in muscle Na-channel mRNA levels, suggesting that expression of Na-channel genes in fast-twitch muscle may be regulated by the state of innervation.

摘要

许多类型的可兴奋细胞中的动作电位是由对钠离子通透性的变化引起的。尽管神经和肌肉中这些通透性的变化是由功能相似的电压门控钠通道介导的，但我们发现骨骼肌中表达的钠通道基因与编码大脑中两种钠通道（I型和II型）的基因不同。尽管肌肉和大脑钠通道基因在结构上存在差异，但从大鼠大脑中获得的一个cDNA克隆与大约9.5千碱基的骨骼肌钠通道mRNA杂交。我们使用这个cDNA探针来测量发育过程中和去神经支配后骨骼肌中钠通道mRNA水平的变化。通过对电泳分离的RNA进行印迹杂交分析，我们发现早在胚胎第17天就可以检测到钠通道mRNA，并且在出生至出生后第35天之间mRNA水平增加了2倍。成年肌肉去神经支配会使肌肉钠通道mRNA水平进一步增加2至3倍，这表明快肌中钠通道基因的表达可能受神经支配状态的调节。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8229/299618/7ec7624df4f4/pnas00338-0559-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8229/299618/d2662e5b16ec/pnas00338-0558-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8229/299618/ea1f5fb4d617/pnas00338-0558-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8229/299618/97002dc1c67e/pnas00338-0558-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8229/299618/40f1c4a219b6/pnas00338-0559-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8229/299618/efb4783121d7/pnas00338-0559-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8229/299618/7ec7624df4f4/pnas00338-0559-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8229/299618/d2662e5b16ec/pnas00338-0558-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8229/299618/ea1f5fb4d617/pnas00338-0558-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8229/299618/97002dc1c67e/pnas00338-0558-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8229/299618/40f1c4a219b6/pnas00338-0559-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8229/299618/efb4783121d7/pnas00338-0559-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8229/299618/7ec7624df4f4/pnas00338-0559-c.jpg

相似文献

1
Modulation of sodium-channel mRNA levels in rat skeletal muscle.大鼠骨骼肌中钠通道mRNA水平的调节
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8721-5. doi: 10.1073/pnas.84.23.8721.
2
Sodium channel distribution in normal and denervated rodent and snake skeletal muscle.正常和去神经支配的啮齿动物及蛇骨骼肌中钠通道的分布
J Physiol. 1988 Jul;401:145-61. doi: 10.1113/jphysiol.1988.sp017155.
3
The glial voltage-gated sodium channel: cell- and tissue-specific mRNA expression.神经胶质电压门控性钠通道：细胞和组织特异性mRNA表达
Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7272-6. doi: 10.1073/pnas.89.15.7272.
4
Expression and distribution of sodium channels in short- and long-term denervated rodent skeletal muscles.钠通道在短期和长期去神经支配的啮齿动物骨骼肌中的表达与分布
J Physiol. 1995 Feb 15;483 ( Pt 1)(Pt 1):109-18. doi: 10.1113/jphysiol.1995.sp020571.
5
Molecular cloning of a putative tetrodotoxin-resistant rat heart Na+ channel isoform.一种假定的抗河豚毒素大鼠心脏钠离子通道亚型的分子克隆
Proc Natl Acad Sci U S A. 1989 Oct;86(20):8170-4. doi: 10.1073/pnas.86.20.8170.
6
Messenger RNA coding for only the alpha subunit of the rat brain Na channel is sufficient for expression of functional channels in Xenopus oocytes.编码大鼠脑钠通道仅α亚基的信使核糖核酸足以在非洲爪蟾卵母细胞中表达功能性通道。
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7503-7. doi: 10.1073/pnas.83.19.7503.
7
Expression of diverse Na+ channel messenger RNAs in rat myocardium. Evidence for a cardiac-specific Na+ channel.大鼠心肌中多种钠离子通道信使核糖核酸的表达。存在心脏特异性钠离子通道的证据。
J Clin Invest. 1989 Jul;84(1):331-6. doi: 10.1172/JCI114158.
8
Single Na+ channel currents observed in cultured rat muscle cells.在培养的大鼠肌肉细胞中观察到的单个钠离子通道电流。
Nature. 1980 Oct 2;287(5781):447-9. doi: 10.1038/287447a0.
9
Myogenin and MyoD join a family of skeletal muscle genes regulated by electrical activity.肌细胞生成素和肌分化抗原属于受电活动调节的骨骼肌基因家族。
Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1349-53. doi: 10.1073/pnas.88.4.1349.
10
Primary structure and expression of a sodium channel characteristic of denervated and immature rat skeletal muscle.去神经支配和未成熟大鼠骨骼肌钠通道的一级结构与表达
Neuron. 1990 Feb;4(2):233-42. doi: 10.1016/0896-6273(90)90098-z.

引用本文的文献

1
Sustained signaling by phospholipase C-gamma mediates nerve growth factor-triggered gene expression.磷脂酶C-γ的持续信号传导介导神经生长因子触发的基因表达。
Mol Cell Biol. 2001 Apr;21(8):2695-705. doi: 10.1128/MCB.21.8.2695-2705.2001.
2
Identification of PN1, a predominant voltage-dependent sodium channel expressed principally in peripheral neurons.PN1的鉴定，PN1是一种主要在外周神经元中表达的电压依赖性钠通道。
Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1527-32. doi: 10.1073/pnas.94.4.1527.
3
GTPase-deficient G alpha 16 and G alpha q induce PC12 cell differentiation and persistent activation of cJun NH2-terminal kinases.

本文引用的文献

1
Measurement of current-voltage relations in the membrane of the giant axon of Loligo.枪乌贼巨大轴突膜电流-电压关系的测量。
J Physiol. 1952 Apr;116(4):424-48. doi: 10.1113/jphysiol.1952.sp004716.
2
The sodium channel from rat brain. Purification and subunit composition.大鼠脑钠通道。纯化及亚基组成。
J Biol Chem. 1984 Feb 10;259(3):1667-75.
3
Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.鉴定出与人类β-干扰素基因相邻的两个不同调控区域。
缺乏GTP酶的Gα16和Gαq诱导PC12细胞分化以及cJun氨基末端激酶的持续激活。
Mol Cell Biol. 1996 Feb;16(2):648-56. doi: 10.1128/MCB.16.2.648.
4
A branched signaling pathway for nerve growth factor is revealed by Src-, Ras-, and Raf-mediated gene inductions.Src、Ras和Raf介导的基因诱导揭示了神经生长因子的分支信号通路。
Mol Cell Biol. 1993 Jun;13(6):3146-55. doi: 10.1128/mcb.13.6.3146-3155.1993.
5
Neuronal growth factor regulation of two different sodium channel types through distinct signal transduction pathways.神经元生长因子通过不同的信号转导途径对两种不同类型的钠通道进行调节。
J Cell Biol. 1993 Aug;122(4):915-21. doi: 10.1083/jcb.122.4.915.
6
Amino acid sequence of a putative sodium channel expressed in the giant axon of the squid Loligo opalescens.在太平洋褶柔鱼巨大轴突中表达的一种假定钠通道的氨基酸序列。
Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10026-30. doi: 10.1073/pnas.90.21.10026.
7
Expression and distribution of sodium channels in short- and long-term denervated rodent skeletal muscles.钠通道在短期和长期去神经支配的啮齿动物骨骼肌中的表达与分布
J Physiol. 1995 Feb 15;483 ( Pt 1)(Pt 1):109-18. doi: 10.1113/jphysiol.1995.sp020571.
8
The tetrodotoxin-insensitive sodium current in rat dorsal root ganglia is unlikely to involve the expression of the tetrodotoxin-resistant sodium channel, SkM2.
Neurochem Res. 1995 Jun;20(6):713-7. doi: 10.1007/BF01705540.
9
Activity-dependent regulation of gene expression in muscle and neuronal cells.肌肉和神经细胞中基因表达的活性依赖性调控。
Mol Neurobiol. 1989 Spring-Summer;3(1-2):1-53. doi: 10.1007/BF02935587.
10
Expression of diverse Na+ channel messenger RNAs in rat myocardium. Evidence for a cardiac-specific Na+ channel.大鼠心肌中多种钠离子通道信使核糖核酸的表达。存在心脏特异性钠离子通道的证据。
J Clin Invest. 1989 Jul;84(1):331-6. doi: 10.1172/JCI114158.
Cell. 1983 Oct;34(3):865-79. doi: 10.1016/0092-8674(83)90544-5.
4
Differences in the properties of Na+ channels in muscle surface and T-tubular membranes revealed by tetrodotoxin derivatives.河豚毒素衍生物揭示的肌肉表面与横管膜中钠离子通道特性的差异
Pflugers Arch. 1983 Apr;397(1):1-5. doi: 10.1007/BF00585159.
5
Na+ channels with high and low affinity tetrodotoxin binding sites in the mammalian skeletal muscle cell. Difference in functional properties and sequential appearance during rat skeletal myogenesis.哺乳动物骨骼肌细胞中具有高亲和力和低亲和力河豚毒素结合位点的钠离子通道。大鼠骨骼肌肌生成过程中功能特性及顺序出现的差异。
J Biol Chem. 1983 Jun 25;258(12):7256-9.
6
Tetrodotoxin-sensitive sodium channels in rat muscle cells developing in vitro.体外培养的大鼠肌肉细胞中的河豚毒素敏感钠通道
J Biol Chem. 1983 Feb 25;258(4):2488-95.
7
Voltage-clamp experiments in normal and denervated mammalian skeletal muscle fibres.正常和去神经支配的哺乳动物骨骼肌纤维中的电压钳实验。
J Physiol. 1980 Sep;306:377-410. doi: 10.1113/jphysiol.1980.sp013403.
8
Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence.从cDNA序列推导的电鳗钠通道一级结构。
Nature. 1984;312(5990):121-7. doi: 10.1038/312121a0.
9
Tetrodotoxin-insensitive sodium channels. Ion flux studies of neurotoxin action in a clonal rat muscle cell line.河豚毒素不敏感的钠通道。在克隆大鼠肌肉细胞系中对神经毒素作用的离子通量研究。
J Biol Chem. 1981 Jun 25;256(12):6213-22.
10
Studies on tetrodotoxin resistant action potentials in denervated skeletal muscle.失神经支配骨骼肌中对河豚毒素有抗性的动作电位的研究。
Acta Physiol Scand. 1971 Nov;83(3):382-8. doi: 10.1111/j.1748-1716.1971.tb05091.x.