College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China.
College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China.
Bioresour Technol. 2014 Mar;155:342-51. doi: 10.1016/j.biortech.2013.12.120. Epub 2014 Jan 8.
The two-phase anaerobic co-digestion of cassava dregs (CD) with pig manure (PM) was evaluated using four sequencing batch reactors (SBRs) and a continuously stirred tank reactor (CSTR). The effect of seven different PM to CD volatile solid ratios (10:0, 8:2, 6:4, 5:5, 4:6, 2:8 and 0:10) on the acidification phase was investigated. Results indicated the concentrations of soluble chemical oxygen demand, NH4-N and volatile fatty acids increased substantially at seven ratios. Co-acidification of PM and CD performed well. Methanogenic fermentation of the acidification products at seven ratios was steady in CSTR. The highest methane yield and VS removal of 0.352m(3)/kg VSadded and 68.5% were achieved at PM:CD (4:6). The microbial population in CSTR was analyzed using molecular methods. Findings revealed that bacteria such as Firmicutes and Bacteroidetes, archaea such as Methanobacteriales and Methanomicrobiales were advantageous populations. Co-digestion of PM and CD supported higher quantity and diversity of methanogens.
采用四个序批式反应器（SBR）和一个连续搅拌罐式反应器（CSTR），评估了木薯渣（CD）与猪粪（PM）的两相厌氧共消化。考察了七种不同 PM 与 CD 挥发性固体比（10:0、8:2、6:4、5:5、4:6、2:8 和 0:10）对酸化阶段的影响。结果表明，在七种比例下，可溶性化学需氧量、NH4-N 和挥发性脂肪酸的浓度均大幅增加。PM 和 CD 的共酸化效果良好。在 CSTR 中，七种比例下的酸化产物的产甲烷发酵均稳定。在 PM:CD（4:6）时，获得了最高的甲烷产率和 VS 去除率，分别为 0.352m³/kg VSadded 和 68.5%。采用分子方法分析了 CSTR 中的微生物种群。结果表明，厚壁菌门和拟杆菌门等细菌、甲烷杆菌目和甲烷微菌目等古菌是优势种群。PM 和 CD 的共消化支持了更多数量和更高多样性的产甲烷菌。
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