Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
Cytotechnology. 2015 Aug;67(4):593-600. doi: 10.1007/s10616-013-9679-7. Epub 2014 Jan 25.
Siglecs, sialic acid-recognizing Ig-superfamily lectins, regulate various aspects of immune responses, and have also been shown to induce the endocytosis of binding materials such as anti-Siglec antibodies or sialic acid-harboring bacteria. In this study, we demonstrated that the expression of Siglec-9 enhanced the transfection efficiency of several cell lines such as macrophage RAW264 and non-hematopoietic 293FT cells. We applied this finding to the production of a lentiviral vector in which cells were transfected simultaneously with multiple vectors, and achieved a twice increase in viral production levels. Furthermore, 293FT cells expressing lectin-defective Siglec-9 produced three- to seven-fold higher titer of viral vector compared with parental 293FT cells. These results suggest that Siglec-9 enhanced lentiviral vector production in a lectin-independent manner.
Siglecs（唾液酸识别 Ig 超家族凝集素）调节免疫反应的各个方面，并且已被证明可以诱导结合物质（如抗 Siglec 抗体或含有唾液酸的细菌）的内吞作用。在这项研究中，我们证明 Siglec-9 的表达增强了几种细胞系（如巨噬细胞 RAW264 和非造血 293FT 细胞）的转染效率。我们将这一发现应用于生产慢病毒载体，其中细胞同时转染多个载体，并使病毒产量水平提高了两倍。此外，表达凝集素缺陷型 Siglec-9 的 293FT 细胞产生的病毒载体滴度比亲本 293FT 细胞高 3 到 7 倍。这些结果表明 Siglec-9 以凝集素非依赖的方式增强了慢病毒载体的产生。
