相关超分辨率荧光和金属复型透射电子显微镜。
Correlative super-resolution fluorescence and metal-replica transmission electron microscopy.
机构信息
1] National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland, USA. [2].
1] Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA. [2].
出版信息
Nat Methods. 2014 Mar;11(3):305-8. doi: 10.1038/nmeth.2816. Epub 2014 Jan 26.
We combine super-resolution localization fluorescence microscopy with transmission electron microscopy of metal replicas to locate proteins on the landscape of the cellular plasma membrane at the nanoscale. We validate robust correlation on the scale of 20 nm by imaging endogenous clathrin (in two and three dimensions) and apply the method to find the previously unknown three-dimensional position of the endocytic protein epsin on clathrin-coated structures at the plasma membrane.
我们将超分辨率定位荧光显微镜与金属复制品的透射电子显微镜相结合，以在纳米尺度上定位细胞质膜表面的蛋白质。我们通过对内源性网格蛋白（二维和三维）进行成像，验证了在 20nm 尺度上可靠的相关性，并将该方法应用于发现内吞蛋白 epsin 在质膜网格蛋白包被结构上以前未知的三维位置。
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