Ebrahimi Loghman, Saboory Ehsan, Roshan-Milani Shiva, Hashemi Paria
Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Dev Psychobiol. 2014 Sep;56(6):1179-86. doi: 10.1002/dev.21198. Epub 2014 Jan 24.
Prenatal exposure to stress and morphine has complicated effects on epileptic seizure. Many reports have shown an interaction between morphine- and stress-induced behavioral changes in adult rats. In the present study, effect of prenatal forced-swim stress and morphine co-administration on pentylentetrazole (PTZ)-induced epileptic behaviors was investigated in rat offspring to address effect of the interaction between morphine and stress. Pregnant rats were divided to four groups of control-saline, control-morphine, stressed-saline and stressed-morphine. In the stressed group, the rats were placed in 25 °C water on 17-19 days of pregnancy. In the morphine/saline group, the rats received morphine/saline on the same days. In the morphine/saline-stressed group, they were exposed to stress and received morphine/saline simultaneously. On postnatal day 15 (P15), blood samples were collected to determine corticosterone (COS) level. On P15 and P25, PTZ was injected to the rest of pups to induce seizure. Then, epileptic behaviors of each rat were individually observed. Latency of tonic-colonic seizures decreased in control-morphine and stressed-saline groups while increasing in stressed-morphine rats compared to control-saline group on P15. Duration of tonic-colonic seizures significantly increased in control-morphine and stressed-saline rats compared to stressed-morphine and control-saline rats on P15, but not P25. COS levels increased in stressed-saline group but decreased in control-morphine group compared to control-saline rats. Body weight was significantly higher in morphine groups than saline treated rats. Prenatal exposure to forced-swim stress potentiated PTZ-induced seizure in the offspring rats. Co-administration of morphine attenuated effect of stress on body weight, COS levels, and epileptic behaviors.
产前暴露于应激和吗啡对癫痫发作有复杂的影响。许多报告显示成年大鼠中吗啡诱导的行为变化与应激诱导的行为变化之间存在相互作用。在本研究中，为了探讨吗啡与应激之间相互作用的影响，研究了产前强迫游泳应激和吗啡联合给药对大鼠后代戊四氮（PTZ）诱导的癫痫行为的影响。将怀孕大鼠分为四组：对照-生理盐水组、对照-吗啡组、应激-生理盐水组和应激-吗啡组。在应激组中，于妊娠第17至19天将大鼠置于25℃的水中。在吗啡/生理盐水组中，大鼠在同一天接受吗啡/生理盐水。在吗啡/生理盐水-应激组中，它们同时接受应激并接受吗啡/生理盐水。在出生后第15天（P15），采集血样以测定皮质酮（COS）水平。在P15和P25时，对其余幼崽注射PTZ以诱导癫痫发作。然后，分别观察每只大鼠的癫痫行为。与对照-生理盐水组相比，在P15时，对照-吗啡组和应激-生理盐水组的强直-阵挛性癫痫发作潜伏期缩短，而应激-吗啡组大鼠的潜伏期延长。在P15时，对照-吗啡组和应激-生理盐水组大鼠的强直-阵挛性癫痫发作持续时间与应激-吗啡组和对照-生理盐水组相比显著增加，但在P25时无此现象。与对照-生理盐水组大鼠相比，应激-生理盐水组的COS水平升高，而对照-吗啡组降低。吗啡组的体重显著高于生理盐水处理组大鼠。产前暴露于强迫游泳应激会增强后代大鼠中PTZ诱导的癫痫发作。吗啡联合给药减弱了应激对体重、COS水平和癫痫行为的影响。
