Gottlieb M, Chavko M
Institute of Neurobiology of Slovak Academy of Sciences, Kosice, Czechoslovakia.
Anal Biochem. 1987 Aug 15;165(1):33-7. doi: 10.1016/0003-2697(87)90197-7.
A modified method of silver staining for native and denatured eucaryotic DNA in 1% agarose gel is described. This method is at least fivefold more sensitive than ethidium bromide staining, with a detection limit of 2.5 ng for total DNA. The calibration curve is linear within the range 5-30 ng of single-stranded and double-stranded DNA. This method is especially advantageous for electrophoretic assessment of DNA molecular weights.
