Skopp R N, Lane L C
Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
Anal Biochem. 1988 Feb 15;169(1):132-7. doi: 10.1016/0003-2697(88)90263-1.
This paper describes agarose gel electrophoresis and silver staining of denatured RNAs. Glyoxal- or formaldehyde-denatured RNAs are electrophoresed in an agarose gel cast on a plastic support using an inert, low conductivity buffer. Following electrophoresis, the gel is stained with a sensitive silver stain. The method produces sharp, well-resolved bands and yields accurate RNA size estimates. Because of its sensitivity and simplicity, it is suitable for routine laboratory use.
本文描述了变性RNA的琼脂糖凝胶电泳和银染方法。用乙二醛或甲醛变性的RNA在以惰性、低电导率缓冲液制备的塑料支持物上浇铸的琼脂糖凝胶中进行电泳。电泳后，凝胶用灵敏的银染剂染色。该方法能产生清晰、分辨率高的条带，并能准确估计RNA大小。由于其灵敏度高且操作简单，适用于常规实验室使用。
