Salvador Soler Noemi, Gómez Garreta Amelia, Ribera Siguan Ma Antonia, Kapraun Donald F
Education Department, Universidad Autónoma de Chile, Temuco, Chile.
Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain.
PLoS One. 2014 Jan 22;9(1):e86006. doi: 10.1371/journal.pone.0086006. eCollection 2014.
Nuclear DNA content in gametophytes and sporophytes or the prostrate phases of the following species of Bonnemaisoniaceae (Asparagopsis armata, Asparagopsis taxiformis, Bonnemaisonia asparagoides, Bonnemaisonia clavata and Bonnemaisonia hamifera) were estimated by image analysis and static microspectrophotometry using the DNA-localizing fluorochrome DAPI (4', 6-diamidino-2-phenylindole, dilactate) and the chicken erythrocytes standard. These estimates expand on the Kew database of DNA nuclear content. DNA content values for 1C nuclei in the gametophytes (spermatia and vegetative cells) range from 0.5 pg to 0.8 pg, and for 2C nuclei in the sporophytes or the prostrate phases range from 1.15-1.7 pg. Although only the 2C and 4C values were observed in the sporophyte or the prostrate phase, in the vegetative cells of the gametophyte the values oscillated from 1C to 4C, showing the possible start of endopolyploidy. The results confirm the alternation of nuclear phases in these Bonnemaisoniaceae species, in those that have tetrasporogenesis, as well as those that have somatic meiosis. The availability of a consensus phylogenetic tree for Bonnemaisoniaceae has opened the way to determine evolutionary trends in DNA contents. Both the estimated genome sizes and the published chromosome numbers for Bonnemaisoniaceae suggest a narrow range of values consistent with the conservation of an ancestral genome.
利用DNA定位荧光染料4',6-二脒基-2-苯基吲哚二盐酸盐（DAPI）和鸡红细胞标准品，通过图像分析和静态显微分光光度法，估算了以下羽藻科物种（武装松节藻、塔形松节藻、龙须菜、棒状松节藻和多叉松节藻）配子体、孢子体或匍匐阶段的核DNA含量。这些估算扩展了英国皇家植物园的DNA核含量数据库。配子体（精子和营养细胞）中1C核的DNA含量值在0.5皮克至0.8皮克之间，孢子体或匍匐阶段2C核的DNA含量值在1.15 - 1.7皮克之间。虽然在孢子体或匍匐阶段仅观察到2C和4C值，但在配子体的营养细胞中，值在1C到4C之间波动，显示出可能开始的核内多倍体现象。结果证实了这些羽藻科物种中核相的交替，包括那些具有四分孢子发生的物种以及那些具有体细胞减数分裂的物种。羽藻科共有系统发育树的可得性为确定DNA含量的进化趋势开辟了道路。羽藻科的估计基因组大小和已公布的染色体数目都表明数值范围较窄，这与祖先基因组的保守性一致。
