Haslin C, Lahaye M, Pellegrini M, Chermann J C
Laboratoire de Biologie Marine Fondamentale et Appliquée, Faculté des Sciences de Luminy, Marseille, France.
Planta Med. 2001 Jun;67(4):301-5. doi: 10.1055/s-2001-14330.
The gametic, carposporic and tetrasporic reproductive stages from the Mediterranean red alga Asparagopsis armata contain peculiar sulfated galactans with galactose:3,6-anhydrogalactose:sulfates molar ratio of 1:0.01:1.23, 1:0.04:0.47 and 1:0.01:1.13, respectively. These water-soluble polysaccharides were studied for their in vitro activity against the human immunodeficiency virus (HIV-1). Gametic and tetrasporic galactans inhibit HIV replication at 10 and 8 micrograms/ml, respectively, as measured by HIV-induced syncitium formation as well as reverse transcriptase activity in cell-free culture supernatant. The carposporic polysaccharide is ineffective, even at 100 micrograms/ml. The maximal antiviral effect involves the presence of the polysaccharides after or during infection but not before infection. This time of action suggests an inhibition of an early step of HIV infection.
来自地中海红藻阿氏藻（Asparagopsis armata）的配子体、果孢子体和四分孢子体繁殖阶段含有特殊的硫酸化半乳聚糖，其半乳糖：3,6-脱水半乳糖：硫酸盐的摩尔比分别为1:0.01:1.23、1:0.04:0.47和1:0.01:1.13。对这些水溶性多糖进行了抗人类免疫缺陷病毒（HIV-1）的体外活性研究。通过HIV诱导的合胞体形成以及无细胞培养上清液中的逆转录酶活性测定，配子体和四分孢子体半乳聚糖分别在10微克/毫升和8微克/毫升时抑制HIV复制。果孢子体多糖即使在100微克/毫升时也无效。最大抗病毒作用涉及多糖在感染后或感染期间存在，但在感染前不存在。这种作用时间表明抑制了HIV感染的早期步骤。
