Zhao Jianyu, Liu Meiling, Jiang Li, Ding Lian, Yan Shuang Shuang, Zhang Juan, Dong Zhaobin, Ren Huazhong, Zhang Xiaolan
Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, P. R. China.
National Maize Improvement Center of China, Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, P. R. China.
PLoS One. 2014 Jan 23;9(1):e86192. doi: 10.1371/journal.pone.0086192. eCollection 2014.
The Arabidopsis SUPERMAN (SUP) gene encodes a C2H2 type zinc finger protein that is required for maintaining the boundaries between stamens and carpels, and for regulating development of ovule outer integument. Orthologs of SUP have been characterized in bisexual flowers as well as dioecious species, but it remains elusive in monoecious plants with unisexual flowers on the same individual. Here we isolate the SUP ortholog in Cucumis sativus L (CsSUP), a monoecious vegetable. CsSUP is predominantly expressed in female specific organs: the female flower buds and ovules. Ectopic expression of CsSUP in Arabidopsis can partially complement the fruit development in sup-5 mutant, and its over-expression in wide-type leads to reduced silique length, suppressed stamen development and distorted petal patterning. Our data suggest that CsSUP plays conserved as well as distinct roles during flower and fruit development, and it may function in the boundaries and ovules to balance petal patterning, stamen and ovule development in Arabidopsis.
拟南芥SUPERMAN（SUP）基因编码一种C2H2型锌指蛋白，该蛋白对于维持雄蕊和心皮之间的界限以及调节胚珠外珠被的发育是必需的。SUP的直系同源基因已在两性花以及雌雄异株物种中得到表征，但在同一植株上具有单性花的雌雄同株植物中仍不清楚。在这里，我们分离出雌雄同株蔬菜黄瓜（Cucumis sativus L）中的SUP直系同源基因（CsSUP）。CsSUP主要在雌性特异器官中表达：雌花芽和胚珠。CsSUP在拟南芥中的异位表达可以部分补充sup-5突变体中的果实发育，并且其在野生型中的过表达导致角果长度缩短、雄蕊发育受抑制以及花瓣图案变形。我们的数据表明，CsSUP在花和果实发育过程中发挥着保守和独特的作用，并且它可能在边界和胚珠中发挥作用，以平衡拟南芥中的花瓣图案、雄蕊和胚珠发育。
