Li Jia, Zhang Litian, Yuan Yibing, Wang Qi, Elbaiomy Rania G, Zhou Wanhai, Wu Hui, Soaud Salma A, Abbas Manzar, Chen Bo, Zhao Deming, El-Sappah Ahmed H
Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, China.
State Key Laboratory of Plateau Ecology and Agriculture, Academy of Animal Science and Veterinary Medicine of Qinghai University, Xining, China.
Front Genet. 2021 Nov 5;12:770427. doi: 10.3389/fgene.2021.770427. eCollection 2021.
The C2H2-zinc finger proteins (ZFP) comprise a large family of transcription factors with various functions in biological processes. In maize, the function regulation of C2H2- zine finger (ZF) genes are poorly understood. We conducted an evolution analysis and functional prediction of the maize C2H2-ZF gene family. Furthermore, the gene has been cloned and sequenced for further favorable allelic variation discovery. The phylogenetic analysis of the C2H2-ZF domain indicated that the position and sequence of the C2H2-ZF domain of the poly-zinc finger gene are relatively conserved during evolution, and the C2H2-ZF domain with the same position is highly conserved. The expression analysis of the C2H2-ZF gene family in 11 tissues at different growth stages of B73 inbred lines showed that genes with multiple transcripts were endowed with more functions. The expression analysis of the C2H2-ZF gene in P1 and P2 inbred lines under drought conditions showed that the C2H2-ZF genes were mainly subjected to negative regulation under drought stress. Functional prediction indicated that the maize C2H2-ZF gene is mainly involved in reproduction and development, especially concerning the formation of important agronomic traits in maize yield. Furthermore, sequencing and correlation analysis of the gene indicated that this gene was significantly associated with the SDW-NAP and TDW-NAP. The analysis of the relationship between maize C2H2-ZF genes and C2H2-ZF genes with known functions indicated that the functions of some C2H2-ZF genes are relatively conservative, and the functions of homologous genes in different species are similar.
C2H2型锌指蛋白（ZFP）构成了一个大型转录因子家族，在生物过程中具有多种功能。在玉米中，人们对C2H2型锌指（ZF）基因的功能调控了解甚少。我们对玉米C2H2-ZF基因家族进行了进化分析和功能预测。此外，该基因已被克隆和测序，以进一步发现有利的等位基因变异。对C2H2-ZF结构域的系统发育分析表明，多锌指基因的C2H2-ZF结构域的位置和序列在进化过程中相对保守，且相同位置的C2H2-ZF结构域高度保守。对B73自交系不同生长阶段11个组织中C2H2-ZF基因家族的表达分析表明，具有多个转录本的基因具有更多功能。对P1和P2自交系在干旱条件下C2H2-ZF基因的表达分析表明，C2H2-ZF基因在干旱胁迫下主要受到负调控。功能预测表明，玉米C2H2-ZF基因主要参与生殖和发育，尤其涉及玉米产量中重要农艺性状的形成。此外，对该基因的测序和相关性分析表明，该基因与地上部干重-吐丝期和总干重-吐丝期显著相关。对玉米C2H2-ZF基因与已知功能的C2H2-ZF基因之间关系的分析表明，一些C2H2-ZF基因的功能相对保守，不同物种中的同源基因功能相似。
