Lee K, McCray W H, Doetsch P W
Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322.
Biochem Biophys Res Commun. 1987 Nov 30;149(1):93-101. doi: 10.1016/0006-291x(87)91609-3.
A thymine glycol-DNA glycosylase/AP endonuclease has been identified in human CEM-C1 lymphoblasts. The enzyme is active in the absence of divalent cations and has an apparent molecular size of approximately 60,000 daltons. The enzyme releases thymine glycol from osmium tetroxide-damaged DNA via an N-glycosylase activity and is associated with an endonuclease activity that mediates phosphodiester bond cleavage at sites of thymine glycol and apurinic sites. We propose that this enzyme, which we call redoxyendonuclease, is the human analog of a bacterial enzyme, E. coli endonuclease III, that recognizes oxidative DNA damage.
在人CEM - C1淋巴母细胞中已鉴定出一种胸腺嘧啶乙二醇 - DNA糖基化酶/AP内切核酸酶。该酶在没有二价阳离子的情况下具有活性，其表观分子大小约为60,000道尔顿。该酶通过N - 糖基化酶活性从四氧化锇损伤的DNA中释放胸腺嘧啶乙二醇，并与一种内切核酸酶活性相关，该活性介导胸腺嘧啶乙二醇和脱嘌呤位点处的磷酸二酯键断裂。我们提出这种酶，我们称之为氧化还原内切核酸酶，是细菌酶大肠杆菌内切核酸酶III的人类类似物，它能识别氧化性DNA损伤。
