Hutt-Taylor S R, Harnish D, Richardson M, Ishizaka T, Denburg J A
McMaster University Medical Centre, Department of Pathology, Hamilton, Ontario, Canada.
Blood. 1988 Jan;71(1):209-15.
Sodium butyrate induces basophilic differentiation of HL-60 promyelocytic leukemia cells that have been previously passaged in alkaline medium. A factor present in Mo conditioned medium (Mo-CM) acts synergistically with sodium butyrate to promote basophilic maturation in a dose-dependent fashion. The induced HL-60 cells exhibit nuclei at various stages of maturity and cytoplasmic granules staining azurophilic with May-Grünwald-Giemsa and metachromatically with toluidine blue. The histamine content of induced HL-60 cells is 50 ng/10(6) cells with sodium butyrate alone or 190 ng/10(6) cells with butyrate in combination with Mo-CM. Induced cells release histamine in response to anti-IgE and have receptors for the Fc portion of human IgE. The basophilic cell-differentiating activity present in Mo-CM appears to be distinct from several other cytokines including recombinant human interleukin-1 alpha, interleukin-2, interferon-gamma, interferon-alpha, murine interleukin-3, erythroid-potentiating activity, and purified human granulocyte/macrophage colony-stimulating factor. This is the first demonstration of a cell line that is capable of differentiation along the basophil lineage and could provide a useful model for examining biochemical and molecular events associated with basophil differentiation.
丁酸钠可诱导先前在碱性培养基中传代的HL-60早幼粒细胞白血病细胞向嗜碱性粒细胞分化。Mo条件培养基（Mo-CM）中存在的一种因子与丁酸钠协同作用，以剂量依赖的方式促进嗜碱性粒细胞成熟。诱导后的HL-60细胞显示出处于不同成熟阶段的细胞核，其细胞质颗粒用May-Grünwald-Giemsa染色呈嗜天青性，用甲苯胺蓝染色呈异染性。单独使用丁酸钠时，诱导后的HL-60细胞组胺含量为50 ng/10⁶细胞；丁酸钠与Mo-CM联合使用时，组胺含量为190 ng/10⁶细胞。诱导后的细胞可响应抗IgE释放组胺，并具有人IgE Fc部分的受体。Mo-CM中存在的嗜碱性粒细胞分化活性似乎与其他几种细胞因子不同，包括重组人白细胞介素-1α、白细胞介素-2、干扰素-γ、干扰素-α、鼠白细胞介素-3、红细胞增强活性和纯化的人粒细胞/巨噬细胞集落刺激因子。这是首次证明一种细胞系能够沿嗜碱性粒细胞谱系分化，可为研究与嗜碱性粒细胞分化相关的生化和分子事件提供有用的模型。
