Walton J E, Hale A S, Brooks M B, Boag A K, Barnett W, Dean R
Pet Blood Bank UK, Loughborough Technology Centre, Loughborough, UK.
J Vet Intern Med. 2014 Mar-Apr;28(2):571-5. doi: 10.1111/jvim.12277. Epub 2014 Jan 27.
Standard practice in canine blood banking is to produce fresh frozen plasma (FFP) by separating and freezing plasma produced from blood within 8 hours of collection. Within canine blood donation programs, this can limit the number of units collected.
HYPOTHESIS/OBJECTIVES: The aim was to compare the coagulation factor and hemostatic protein content (CF&HPC) of plasma produced from blood stored at ambient temperature for 8, 12, and 24 hours. Another aim was to compare the CF&HPC between Greyhound types and other breeds.
None.
In vitro study. A convenience sample of 58 units of canine blood from a blood donor pool was processed to prepare and freeze plasma 8, 12, or 24 hours following collection.
Regardless of time of processing, the units contained therapeutic CF&HPC. Frozen plasma prepared after 24 hours had significantly higher factor VIII (P = .014) and factor X (P = .03) when compared with the frozen plasma prepared at 8 hours. Factor X (P < .01), fibrinogen (P < .01), and vWF (P = .04) were significantly lower in plasma collected from Greyhound types than in plasma collected from other breeds.
Storing whole blood for up to 24 hours is a suitable method for producing FFP. Lower values for some coagulation factors and hemostatic proteins in plasma produced from Greyhound types would not preclude these dogs as FFP donors.
犬类血液库的标准做法是通过分离并冷冻采集后8小时内血液产生的血浆来制备新鲜冷冻血浆（FFP）。在犬类献血项目中，这可能会限制采集的单位数量。
假设/目标：目的是比较在室温下储存8小时、12小时和24小时的血液所产生的血浆的凝血因子和止血蛋白含量（CF&HPC）。另一个目的是比较灵缇犬类型和其他品种之间的CF&HPC。
无。
体外研究。从一个献血者库中选取58单位犬类血液的便利样本，在采集后8小时、12小时或24小时进行处理以制备并冷冻血浆。
无论处理时间如何，这些单位都含有治疗性CF&HPC。与8小时制备的冷冻血浆相比，24小时后制备的冷冻血浆的凝血因子VIII（P = 0.014）和凝血因子X（P = 0.03）显著更高。灵缇犬类型采集的血浆中的凝血因子X（P < 0.01）、纤维蛋白原（P < 0.01）和血管性血友病因子（vWF）（P = 0.04）显著低于其他品种采集的血浆。
将全血储存长达24小时是制备FFP的合适方法。灵缇犬类型产生的血浆中某些凝血因子和止血蛋白的值较低并不排除这些犬作为FFP献血者。
