Manenti G, Dragani T A, Della Porta G
Division of Experimental Oncology A, Istituto Nazionale Tumori, Milano, Italy.
Chem Biol Interact. 1987;64(1-2):83-92. doi: 10.1016/0009-2797(87)90062-7.
The promoters of murine hepatocarcinogenesis phenobarbital (PB) and 1,4-bis[2-(3,5-dichloropyridyloxy)]benzene (TCPOBOP) given to adult C3Hf female mice increased the content of total liver DNA by 1.6-1.8-fold each week after the beginning of treatment. Both compounds increased the aminopyrine-N-demethylase activity, decreased the glucose 6-phosphatase (G6Pase), alkaline phosphodiesterase I and alkaline phosphatase specific activities, but did not modify the gamma-glutamyltransferase levels. Both compounds decreased the abundance of tyrosine aminotransferase- and metallothionein I-related RNA transcripts. These findings confirmed the PB-like activity of TCPOBOP and showed that both chemicals had a pleiotropic effect on mouse liver, that was not limited to stimulation of drug metabolism, but also affected other hepatocyte functions.
给成年C3Hf雌性小鼠施用小鼠肝癌发生促进剂苯巴比妥（PB）和1,4-双[2-（3,5-二氯吡啶氧基）]苯（TCPOBOP）后，从治疗开始每周肝脏总DNA含量增加1.6至1.8倍。两种化合物均增加氨基比林-N-脱甲基酶活性，降低葡萄糖6-磷酸酶（G6Pase）、碱性磷酸二酯酶I和碱性磷酸酶的比活性，但不改变γ-谷氨酰转移酶水平。两种化合物均降低酪氨酸转氨酶和金属硫蛋白I相关RNA转录本的丰度。这些发现证实了TCPOBOP具有PB样活性，并表明这两种化学物质对小鼠肝脏具有多效性作用，不仅限于刺激药物代谢，还影响其他肝细胞功能。
