通过高效液相色谱法测定糖尿病患者血浆中的3-羟基-3-甲基戊二酸、己二酸和2-氧代戊二酸。

3-Hydroxy-3-methylglutaric, adipic, and 2-oxoglutaric acids measured by HPLC in the plasma from diabetic patients.

作者信息

Lippe G, Trevisan R, Nosadini R, Fabris R, Deana R

机构信息

Institute of Biochemistry, University of Padova, Italy.

出版信息

Clin Biochem. 1987 Aug;20(4):275-9. doi: 10.1016/s0009-9120(87)80012-7.

DOI:10.1016/s0009-9120(87)80012-7

PMID:2446804

Abstract

A method for the measurement of organic acids in human plasma is presented. The analytical procedure consists of plasma protein precipitation with acetonitrile, acid extraction by chromatography through a DEAE-cellulose column eluted with 100 mM perchloric acid, HPLC by cation-exchange column Aminex HPX-87 eluted with 6.5 mM sulfuric acid. Adipic, 3-hydroxy-3-methylglutaric, 2-oxoglutaric, and citric acids were determined in the plasma of diabetic patients. The concentrations of all the measured acids, but particularly those of adipic and 3-hydroxy-3-methylglutaric acids, were significantly higher than those of healthy controls. These results suggest that in diabetics the omega-oxidation of fatty acids is enhanced.

摘要

本文介绍了一种测量人体血浆中有机酸的方法。分析过程包括用乙腈沉淀血浆蛋白，通过用100 mM高氯酸洗脱的DEAE-纤维素柱进行色谱酸萃取，用6.5 mM硫酸洗脱的阳离子交换柱Aminex HPX-87进行高效液相色谱分析。在糖尿病患者的血浆中测定了己二酸、3-羟基-3-甲基戊二酸、2-氧代戊二酸和柠檬酸。所有测量酸的浓度，尤其是己二酸和3-羟基-3-甲基戊二酸的浓度，均显著高于健康对照组。这些结果表明，糖尿病患者中脂肪酸的ω-氧化增强。