State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Talanta. 2014 Mar;120:376-85. doi: 10.1016/j.talanta.2013.12.004. Epub 2013 Dec 18.
The protein imprinted technique combining surface imprinting and nano-sized supports materials is an attractive strategy for protein recognition and rapid separation. In this work, we imprinted bovine hemoglobin (BHb) on magnetic nanoparticles. With itaconic acid (IA) and acrylamide (AAm) as the monomers, the experiment was carried out in aqueous media via surface-imprinting technique. The effects of initial concentration and adsorption time over the adsorption capacity of both imprinted and non-imprinted nanoparticles were analyzed. The maximum adsorption capability of imprinted nanoparticles was found to be 77.6 mg g(-1), which was 3.1-4.3 times higher than that of the non-imprinted nanoparticles prepared at the same conditions. This resulted in the successful formation of imprinting cavities. Moreover, in selective adsorption experiment and competitive batch rebinding test, imprinted nanoparticles exhibited a high specific recognition of the template protein over the non-imprinted protein.
蛋白质印迹技术结合表面印迹和纳米级支撑材料是一种用于蛋白质识别和快速分离的有吸引力的策略。在这项工作中，我们在磁性纳米粒子上印迹牛血红蛋白（BHb）。以衣康酸（IA）和丙烯酰胺（AAm）为单体，通过表面印迹技术在水介质中进行实验。分析了初始浓度和吸附时间对印迹和非印迹纳米粒子吸附容量的影响。印迹纳米粒子的最大吸附能力为 77.6 mg g(-1)，比在相同条件下制备的非印迹纳米粒子高 3.1-4.3 倍。这导致印迹腔的成功形成。此外，在选择性吸附实验和竞争性批量再结合实验中，印迹纳米粒子对模板蛋白表现出高于非印迹蛋白的高特异性识别。
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