State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
Analyst. 2013 Jan 21;138(2):651-8. doi: 10.1039/c2an36313e.
Surface molecular imprinting, especially on the surface of silica-modified magnetic nanoparticles, has been proposed as a promising strategy for protein recognition and separation. Inspired by the self-polymerization of dopamine, we synthesized a polydopamine-based molecular imprinted film coating on silica-Fe(3)O(4) nanoparticles for recognition and separation of bovine hemoglobin (BHb). Magnetic molecularly imprinted nanoparticles (about 860 nm) possess a core-shell structure. Magnetic molecularly imprinted nanoparticles (MMIP) show a relatively high adsorption capacity (4.65 ± 0.38 mg g(-1)) and excellent selectivity towards BHb with a separation factor of 2.19. MMIP with high saturation magnetization (10.33 emu g(-1)) makes it easy to separate the target protein from solution by an external magnetic field. After three continuous adsorption and elution processes, the adsorption capacity of MMIP remained at 4.30 mg g(-1). Our results suggest that MMIPs are suitable for the removal of high abundance of protein and the enrichment of low abundance of protein in proteomics.
表面分子印迹技术，特别是在经过硅烷化修饰的磁性纳米粒子表面的分子印迹技术，已被提议作为一种有前途的蛋白质识别和分离策略。受多巴胺自聚合的启发，我们合成了一种基于聚多巴胺的分子印迹薄膜，将其涂覆在硅烷化-Fe(3)O(4)纳米粒子上，用于识别和分离牛血红蛋白（BHb）。磁性分子印迹纳米粒子（约 860nm）具有核壳结构。磁性分子印迹纳米粒子（MMIP）对 BHb 表现出较高的吸附容量（4.65±0.38mg/g）和出色的选择性，分离因子为 2.19。MMIP 具有较高的饱和磁化强度（10.33emu/g），使其可以通过外加磁场轻松地从溶液中分离目标蛋白质。经过三个连续的吸附和洗脱过程后，MMIP 的吸附容量仍保持在 4.30mg/g。我们的研究结果表明，MMIP 适合用于去除蛋白质中的高丰度蛋白质和富集蛋白质组学中的低丰度蛋白质。
