Li Lin, He Xiwen, Chen Langxing, Zhang Yukui
Department of Chemistry, Nankai University, Tianjin 300071, PR China
Chem Asian J. 2009 Feb 2;4(2):286-93. doi: 10.1002/asia.200800300.
Imprinting nanoparticles: Core-shell bovine hemoglobin (BHb) imprinted magnetic nanoparticles (MNPs) with a mean diameter of 210 nm have been synthesized for the first time. The imprinted magnetic nanoparticles could easily reach the adsorption equilibrium and magnetic separation under an external magnetic field, thus avoiding problems related to the bulk polymer. In this work, the core-shell bovine hemoglobin (BHb) imprinted magnetic nanoparticles (MNPs) with a mean diameter of 210 nm were synthesized for the first time. In this protocol, the initial step involved co-precipitation of Fe(2+) and Fe(3+) in an ammonia solution. Silica was then coated on the Fe(3)O(4) nanoparticles using a sol-gel method to obtain silica shell magnetic nanoparticles. Subsequently, 3-aminophenylboronic acid (APBA), which is the functional and cross-linking monomer, and poly(APBA) thin films were coated onto the silica-modified Fe(3)O(4) surface through oxidation with ammonium persulfate in an aqueous solution in the presence or absence of protein. The morphology, adsorption, and recognition properties of the magnetic molecularly imprinted nanomaterial were investigated by transmission electron microscopy (TEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM). Rebinding experiments were carried out to establish the equilibrium time and to determine the specific binding capacity and selective recognition. The protein adsorption results showed that poly(APBA) MIPs-coated magnetic nanoparticles have high adsorption capacity for template protein BHb and comparatively low non-specific adsorption. The imprinted magnetic nanoparticles could easily reach the adsorption equilibrium and magnetic separation under an external magnetic field, thus avoiding problems related to the bulk polymer. We believe that the imprinted polymer-coated magnetic nanoparticles can be one of the most promising candidates for various applications, which include chemical and biochemical separation, cell sorting, recognition elements in biosensors, and drug delivery.
首次合成了平均直径为210 nm的核壳型牛血红蛋白（BHb）印迹磁性纳米颗粒（MNPs）。印迹磁性纳米颗粒能够在外部磁场作用下轻松达到吸附平衡并实现磁分离，从而避免了与本体聚合物相关的问题。在本研究中，首次合成了平均直径为210 nm的核壳型牛血红蛋白（BHb）印迹磁性纳米颗粒（MNPs）。在此方案中，第一步是在氨溶液中使Fe(2+)和Fe(3+)共沉淀。然后采用溶胶-凝胶法在Fe(3)O(4)纳米颗粒上包覆二氧化硅，以获得二氧化硅壳磁性纳米颗粒。随后，在有或无蛋白质存在的情况下，通过在水溶液中用过硫酸铵氧化，将作为功能和交联单体的3-氨基苯硼酸（APBA）以及聚（APBA）薄膜包覆在二氧化硅改性的Fe(3)O(4)表面。通过透射电子显微镜（TEM）、X射线衍射（XRD）、热重分析（TGA）和振动样品磁强计（VSM）研究了磁性分子印迹纳米材料的形态、吸附和识别性能。进行了再结合实验以确定平衡时间，并测定特异性结合容量和选择性识别能力。蛋白质吸附结果表明，聚（APBA）MIPs包覆的磁性纳米颗粒对模板蛋白BHb具有高吸附容量且非特异性吸附相对较低。印迹磁性纳米颗粒能够在外部磁场作用下轻松达到吸附平衡并实现磁分离，从而避免了与本体聚合物相关的问题。我们认为，印迹聚合物包覆的磁性纳米颗粒可能是各种应用中最有前景的候选材料之一，这些应用包括化学和生化分离、细胞分选、生物传感器中的识别元件以及药物递送。
