State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, No. 865, Changning Road, Shanghai 200050, China; University of Chinese Academy of Sciences, No. 19A, Yuquan Road, Beijing 100049, China.
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, No. 865, Changning Road, Shanghai 200050, China.
Talanta. 2014 Mar;120:462-9. doi: 10.1016/j.talanta.2013.11.013. Epub 2013 Nov 11.
We present a new cysteamine (CS)-modified polyaniline (PANI) film for highly efficient immobilization of biomolecules in biosensing technology. This electrochemical deposited PANI film treated with CS and glutaraldehyde could be employed as an excellent substrate for biomolecules immobilization. The parameters of PANI growth were optimized to obtain suitable surface morphology of films for biomolecules combination with the help of electron and atomic force microscopy. Cyclic voltammetry (CV) was utilized to illustrate the different electrochemical activities of each modified electrode. Due to the existence of sulfydryl group and amino group in CS, surface modification with CS was proven to reduce oxidized units on PANI film remarkably, as evidenced by both ATR-FTIR and Raman spectroscopy characterizations. Furthermore, bovine serum albumin (BSA) was used as the model protein to investigate the immobilization efficiency of biomolecules on the PANI film, comparative study using quartz crystal microbalance (QCM) showed that BSA immobilized on CS-modified PANI could be increased by at least 20% than that without CS-modified PANI in BSA solution with the concentration of 0.1-1mg/mL. The CS-modified PANI film would be significant for the immobilization and detection of biomolecules and especially promising in the application of immunosensor for ultrasensitive detection.
我们提出了一种新的半胱胺（CS）修饰的聚苯胺（PANI）膜，用于生物传感技术中生物分子的高效固定化。这种经 CS 和戊二醛处理的电化学沉积 PANI 膜可用作生物分子固定化的优异基底。通过电子和原子力显微镜的帮助，优化了 PANI 生长的参数，以获得适合生物分子结合的膜的合适表面形貌。循环伏安法（CV）用于说明每个修饰电极的不同电化学活性。由于 CS 中存在巯基和氨基，表面修饰 CS 被证明可以显著减少 PANI 膜上的氧化单元，这一点通过 ATR-FTIR 和拉曼光谱表征得到了证明。此外，牛血清白蛋白（BSA）被用作模型蛋白来研究生物分子在 PANI 膜上的固定化效率，使用石英晶体微天平（QCM）的对比研究表明，在浓度为 0.1-1mg/mL 的 BSA 溶液中，BSA 固定在 CS 修饰的 PANI 上的效率至少比没有 CS 修饰的 PANI 提高了 20%。CS 修饰的 PANI 膜将对生物分子的固定化和检测具有重要意义，尤其在用于超灵敏检测的免疫传感器应用中具有广阔的前景。
