Chen Chien-Chung, Gu Yesong
Department of Chemical Engineering, Tunghai University, Taichung, Taiwan, ROC.
Biosens Bioelectron. 2008 Jan 18;23(6):765-70. doi: 10.1016/j.bios.2007.08.014. Epub 2007 Aug 23.
Polyaniline (PANI) is considered as one of the most fascinating conductive polymers in fabricating enzyme-based biosensors. Nevertheless, to improve both sensitivity and stability of the PANI-modified biosensor has been and continues to be a technical challenge. In this study, we have electrochemically synthesized the PANI film on a platinum (Pt) electrode and then used this electrode to construct a horseradish peroxidase (HRP)-based biosensor for the detection of hydrogen peroxide (H(2)O(2)). The electrochemical and structural properties of electrodes were characterized with scanning electron microscopy (SEM), thermogravimetric analysis (TGA), Fourier-transform infrared (FTIR) spectrophotometer, and cyclic voltammetry (CV). It was interestingly found that the PANI film synthesized in the presence of bovine serum albumin (BSA) has provided the electrode with enhanced sensitivity and excellent stability. Our results suggested that the embedded BSA might serve as an initial template for aniline polymerization and stabilized the microstructure of the PANI film significantly. The constructed HRP/PANI(BSA)/Pt electrode also exhibited a fine linear correlation with H(2)O(2) concentration. This approach by implanted BSA was useful for improving the sensitivity and stability of PANI-modified biosensor.
聚苯胺（PANI）被认为是制造基于酶的生物传感器时最具吸引力的导电聚合物之一。然而，提高聚苯胺修饰生物传感器的灵敏度和稳定性一直是并将继续是一项技术挑战。在本研究中，我们在铂（Pt）电极上电化学合成了聚苯胺膜，然后使用该电极构建了一种基于辣根过氧化物酶（HRP）的生物传感器，用于检测过氧化氢（H₂O₂）。用电扫描电子显微镜（SEM）、热重分析（TGA）、傅里叶变换红外（FTIR）分光光度计和循环伏安法（CV）对电极的电化学和结构性质进行了表征。有趣的是发现，在牛血清白蛋白（BSA）存在下合成的聚苯胺膜使电极具有更高的灵敏度和出色的稳定性。我们的结果表明，嵌入的BSA可能作为苯胺聚合的初始模板，并显著稳定了聚苯胺膜的微观结构。构建的HRP/PANI(BSA)/Pt电极对H₂O₂浓度也表现出良好的线性相关性。这种通过植入BSA的方法对于提高聚苯胺修饰生物传感器的灵敏度和稳定性是有用的。
