Department of Geography & Environmental Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218-2686, USA.
Department of Chemical & Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218-2686, USA.
Bioresour Technol. 2014 Mar;155:366-72. doi: 10.1016/j.biortech.2013.12.095. Epub 2014 Jan 7.
The effect of various pretreatment strategies on methane yields following anaerobic digestion (AD) of five different microalgal strains was investigated. Pavlova_cf sp., Tetraselmis sp. and Thalassiosira weissflogii exhibited substantial methane yields of 0.4-0.5L/g volatile solids (VS) without pretreatment, providing up to 75-80% of theoretical values. In contrast, methane yields from Chlorella sp. and Nannochloropsis sp. were around 0.35L/g VS, or 55-60% of the theoretical values, respectively. Alkali treatment was not effective and thermal pretreatment only enhanced Nannochloropsis methane yields. Thermochemical pretreatment had the strongest impact on biomass solubilization with methane yields increasing by 30% and 40% for Chlorella and Nannochloropsis, respectively. The lipid content had a strong beneficial impact on the theoretical and observed methane yields as compared to protein and carbohydrate content. Other features such as cell-wall composition are also likely to be important factors dictating algal biodegradability and methane yields addressed in part by thermochemical pretreatment.
研究了五种不同微藻菌株在厌氧消化（AD）前不同预处理策略对甲烷产量的影响。Pavlova_cf sp.、Tetraselmis sp. 和 Thalassiosira weissflogii 未经预处理即可产生 0.4-0.5L/g 挥发性固体（VS）的大量甲烷，达到理论值的 75-80%。相比之下，Chlorella sp. 和 Nannochloropsis sp. 的甲烷产量约为 0.35L/g VS，分别为理论值的 55-60%。碱处理无效，热预处理仅提高了 Nannochloropsis 的甲烷产量。热化学预处理对生物质的溶解有最强的影响，Chlorella 和 Nannochloropsis 的甲烷产量分别增加了 30%和 40%。与蛋白质和碳水化合物含量相比，脂质含量对理论和观察到的甲烷产量有很强的有益影响。细胞壁组成等其他特征也可能是决定藻类生物降解性和甲烷产量的重要因素，部分通过热化学预处理来解决。
