培养的鸡胚细胞在高渗处理过程中核糖体RNA、多聚腺苷酸加尾RNA以及胶原蛋白α2(I)信使核糖核酸合成与加工的变化。

Changes in ribosomal RNA, poly(A)+ RNA, and collagen alpha 2(I) mRNA synthesis and processing during hypertonic treatment of cultured chick embryo cells.

作者信息

Gann K L, Lukens L N, Pawlowski P J

机构信息

Department of Biology, Wesleyan University, Middletown, Connecticut 06457.

出版信息

Exp Cell Res. 1988 Jan;174(1):188-98. doi: 10.1016/0014-4827(88)90154-1.

DOI:10.1016/0014-4827(88)90154-1

PMID:2446898

Abstract

The effect of hypertonic conditions on RNA synthesis in cultured chick embryo cells was examined. The appearance of newly synthesized 28 S, 18 S, and 4 S and 5 S RNA into the cytoplasm was found to be decreased by hypertonic conditions. The appearance of newly synthesized poly(A)+ RNA into the cytoplasm was also found to be depressed. To examine the behavior of a specific mRNA, nuclear and cytoplasmic levels of procollagen alpha 2(I) mRNA were measured during high salt treatment. While nuclear levels of this mRNA were found to increase, those of the cytoplasm fell markedly. S1 nuclease digestion studies of an intron flanked by two exons revealed that the pro alpha 2(I) collagen nuclear RNA that accumulated under hypertonic conditions was spliced. The nuclear accumulation of mRNA appears therefore to be due to a hypertonic block of nuclear-cytoplasmic transport, and not to an inhibition of RNA splicing.

摘要

研究了高渗条件对培养的鸡胚细胞中RNA合成的影响。发现高渗条件会使新合成的28 S、18 S、4 S和5 S RNA进入细胞质的量减少。新合成的聚腺苷酸（poly(A)+）RNA进入细胞质的量也被发现受到抑制。为了检测特定mRNA的行为，在高盐处理过程中测量了原胶原蛋白α2（I）mRNA的核内和细胞质水平。虽然发现该mRNA的核内水平增加，但细胞质水平却显著下降。对由两个外显子侧翼的内含子进行S1核酸酶消化研究表明，在高渗条件下积累的原α2（I）胶原蛋白核RNA是经过剪接的。因此，mRNA的核内积累似乎是由于核质运输的高渗阻滞，而不是RNA剪接的抑制。