Pawlowski P J
Biochemistry. 1982 Jan 5;21(1):34-8. doi: 10.1021/bi00530a006.
The major type of collagen synthesized by fibroblasts or bone cells, type I collagen, consists of two chains normally found in a 2:1 ratio designated alpha 1(I)2 alpha 2(I) or more simply alpha 1(I)2 alpha 2. I have analyzed the relative synthesis of type I chains in these cells under conditions which reduce the initiation of protein synthesis. It was found that in bone cells, which make a large amount of collagen, the alpha 1(I):alpha 2 ratio is unaltered whereas in fibroblasts, which make smaller amounts of collagen, the alpha 2 chain is particularly sensitive to these same conditions. Examination of the collagen secreted into the medium, under these same conditions, also revealed an altered chain ratio from cells making low amounts of collagen.
成纤维细胞或骨细胞合成的主要胶原蛋白类型，即I型胶原蛋白，由两条链组成，通常以2:1的比例存在，称为α1(I)2α2(I)，或更简单地称为α1(I)2α2。我分析了在降低蛋白质合成起始的条件下，这些细胞中I型链的相对合成情况。结果发现，在大量合成胶原蛋白的骨细胞中，α1(I):α2的比例未发生改变，而在合成较少胶原蛋白的成纤维细胞中，α2链对这些相同条件特别敏感。在相同条件下，对分泌到培养基中的胶原蛋白进行检测，也发现来自合成少量胶原蛋白的细胞的链比例发生了改变。
