Bioprocessing And Nano-Enzyme Formulation Facility (BANEFF), INRS-ETE, Université du Québec, 490 Rue de la Couronne, Québec, QC, G1K 9A9, Canada.
Appl Biochem Biotechnol. 2014 Mar;172(6):2974-88. doi: 10.1007/s12010-014-0739-z. Epub 2014 Jan 28.
The present work explores brewery wastewater as a novel substrate for fumaric acid production employing the filamentous fungal strain Rhizopus oryzae 1526 through submerged fermentation. The effects of different parameters such as substrate total solid concentrations, fermentation pH, incubation temperature, flask shaking speed, and inoculum size on the fungal morphologies were investigated. Different morphological forms (mycelium clumps, suspended mycelium, and solid/hairy pellets) of R. oryzae 1526 were obtained at different applied fermentation pH, incubation temperature, flask shaking speed, and inoculum size. Among all the obtained morphologies, pellet morphology was found to be the most favorable for enhanced production of fumaric acid for different studied parameters. Scanning electron microscopic investigation was done to reveal the detailed morphologies of the pellets formed under all optimized conditions. With all the optimized growth conditions (pH 6, 25 °C, 200 rpm, 5% (v/v) inoculum size, 25 g/L total solid concentration, and pellet diameter of 0.465 ± 0.04 mm), the highest concentration of fumaric acid achieved was 31.3 ± 2.77 g/L. The results demonstrated that brewery wastewater could be used as a good substrate for the fungal strain R. oryzae 1526 in submerged fermentation for the production of fumaric acid.
本工作探讨了利用丝状真菌米根霉 1526 通过深层发酵，以啤酒废水为新型底物生产富马酸。考察了不同参数，如基质总固体浓度、发酵 pH 值、培养温度、摇瓶转速和接种量对真菌形态的影响。在不同的应用发酵 pH 值、培养温度、摇瓶转速和接种量下，获得了不同的形态形式（菌丝团、悬浮菌丝和固体/多毛颗粒）。在所有获得的形态中，发现颗粒形态最有利于在不同研究参数下增强富马酸的生产。进行了扫描电子显微镜研究，以揭示在所有优化条件下形成的颗粒的详细形态。在所有优化的生长条件下（pH 值 6、25°C、200 rpm、5%（v/v）接种量、25 g/L 总固体浓度和颗粒直径为 0.465±0.04 mm），富马酸的最高浓度达到 31.3±2.77 g/L。结果表明，啤酒废水可作为米根霉 1526 在深层发酵生产富马酸中的良好底物。
