Matthes G, Hübner U, Gränz M, Schütt M, Hackensellner H A
Institut für Transfusiologie und Transplantologie, Humboldt-Universität zu Berlin, GDR.
Folia Haematol Int Mag Klin Morphol Blutforsch. 1987;114(4):482-3.
The influence of antioxidative drugs on the cryopreservation of human bone marrow cells was studied. The viability of bone marrow cells was tested after freezing and thawing from--196 degrees C by the growth of GM-CFC in agar culture. The results suggest the ability of antioxidants to protect stem cells against damage caused by freezing and thawing. Addition of tocopherol or penicillin to a cryoprotective medium increases the number of surviving GM-CFC. From the colony-to-cluster ratio can be concluded that antioxidants especially protect more immature haematopoietic (colony forming) cells.
研究了抗氧化药物对人骨髓细胞冷冻保存的影响。通过在琼脂培养中培养粒-巨噬细胞集落形成细胞（GM-CFC），检测了骨髓细胞在从-196℃冻融后的活力。结果表明抗氧化剂有能力保护干细胞免受冻融损伤。在冷冻保护培养基中添加生育酚或青霉素可增加存活的GM-CFC数量。从集落与集簇比例可以得出结论，抗氧化剂尤其能保护更多未成熟的造血（集落形成）细胞。
