Matthes G, Hübner U, Gränz M, Schütt M
Institute of Transfusiology and Transplantology, Medical School (Charité), Humboldt University, Berlin.
Folia Haematol Int Mag Klin Morphol Blutforsch. 1989;116(3-4):451-4.
Addition of antioxidants into the preservation solution improved the cryoprotection of human bone marrow cells. The viability was studied by the growth of GM-CFC in agar culture before and after storage at -196 degrees C. All used antioxidative drugs (selenomethionine, methionine, tocopherol, penicillin/Fe++) increased the tolerance of the stem cells to freezing and thawing and elevated the number of surviving GM-CFC up to the twofold in comparison with that of controls. More immature colony forming cells were especially protected.
