Service de Biochimie, Departement de Biologie, C.E.N. de SACLAY, BP no2, Gif-sur-Yvette, 91 190, France.
Photosynth Res. 1980 Mar;1(1):3-16. doi: 10.1007/BF00020071.
In thylakoid membranes, several peptides of high MW† are present which may interfere with the study of CP1's components. Modifying Cleveland's technique [7] for limited proteolysis, we have characterized the polypeptides found in the 60 kD region. Some may result from incomplete washing of the CF1 while others come from the CP1; indeed, this chlorophyll protein complex, which has a higher MW (above 100 kD), very often undergoes a dissociation into smaller components of about 60 KD MW.Analysis of the protein content of different preparations commonly used to obtain PSI reaction centre enriched fractions has been performed. The α and β subunits of CF1 are among the main contaminants of most of these preparations. A further purification step is described which can be applied to all these preparations, but numerous peptides are still present in the active fractions. It is most unlikely that all these polypeptides are required for the primary photochemical event, and this emphasizes the necessity to find a new simple method to purify PSI reaction centres.
在类囊体膜中，存在几种高 MW†的肽，这可能会干扰 CP1 成分的研究。我们对克利夫兰的有限蛋白水解技术[7]进行了修改，对在 60 kD 区域发现的多肽进行了表征。其中一些可能是由于 CF1 洗涤不彻底，而另一些则来自 CP1；事实上，这种叶绿素蛋白复合物的 MW 较高（超过 100 kD），经常会发生解离，形成约 60 KD MW 的较小组件。对常用于获得 PSI 反应中心富集部分的不同制剂的蛋白质含量进行了分析。CF1 的 α 和 β 亚基是这些制剂中大多数的主要污染物。本文描述了一种适用于所有这些制剂的进一步纯化步骤，但在活性部分仍存在许多肽。这些多肽不太可能都需要进行初级光化学事件，这强调了需要找到一种新的简单方法来纯化 PSI 反应中心。
