Departmetn de Biologie, C.E.N. Saclay, 91 191, Gif-sur-Yvette, France.
Photosynth Res. 1981 Sep;2(3):195-202. doi: 10.1007/BF00032358.
Spinach CP1 complex, purified as previously described [16], was submitted to various dissociating treatments. Chaotropic agents, like urea and thiocyanate salts, remained without effect on the structure and photooxidation of the complex, just SDS at very high concentrations was able to dissociate the chlorophyll from the polypeptides and to abolish the photoreaction. Proteolytic enzymes have no more action on the apparent structure and activity of CP1, but some of them do cleave the large polypeptides (65 kD) into smaller ones, as observed after pigments dissociation. This last result might be an important step in the search for a smaller active P700 protein complex.
菠菜 CP1 复合物，如前所述 [16] 进行纯化，然后接受各种解离处理。变性剂，如尿素和硫氰酸盐，对复合物的结构和光氧化没有影响，只有在非常高的 SDS 浓度下才能将叶绿素从多肽中解离出来，并消除光反应。蛋白酶对 CP1 的表观结构和活性没有更多作用，但其中一些可以将大的多肽（65 kD）切成较小的多肽，如在色素解离后观察到的那样。这最后一个结果可能是寻找更小的活性 P700 蛋白复合物的重要步骤。
