Tang Yong, Tian Xiuchun Cindy
Center for Regenerative Biology; Department of Animal Science; University of Connecticut; Storrs, CT USA.
JAKSTAT. 2013 Oct 1;2(4):e24935. doi: 10.4161/jkst.24935. Epub 2013 May 7.
Reprogramming somatic cells to pluripotency, especially by the induced pluripotent stem cell (iPSC) technology, has become widely used today to generate various types of stem cells for research and for regenerative medicine. However the mechanism(s) of reprogramming still need detailed elucidation, including the roles played by the leukemia inhibitory factor (LIF) signaling pathway. LIF is central in maintaining the ground state pluripotency of mouse embryonic stem cells (ESCs) and iPSCs by activating the Janus kinase-signal transducer and activator of transcription 3 (JAK-STAT3) pathway. Characterizing and understanding this pathway holds the key to generate naïve pluripotent human iPSCs which will facilitate the development of patient-specific stem cell therapy. Here we review the historical and recent developments on how LIF signaling pathway regulates ESC pluripotency maintenance and somatic cell reprogramming, with a focus on JAK-STAT3.
将体细胞重编程为多能性，尤其是通过诱导多能干细胞（iPSC）技术，如今已被广泛用于生成各种类型的干细胞用于研究和再生医学。然而，重编程的机制仍需要详细阐明，包括白血病抑制因子（LIF）信号通路所起的作用。LIF通过激活Janus激酶-信号转导和转录激活因子3（JAK-STAT3）通路，在维持小鼠胚胎干细胞（ESC）和iPSC的基础多能性方面起着核心作用。表征和理解这条通路是生成原始多能性人类iPSC的关键，这将有助于患者特异性干细胞治疗的发展。在此，我们回顾了LIF信号通路如何调节ESC多能性维持和体细胞重编程的历史及近期进展，重点关注JAK-STAT3。
